User:Eric Ma/Notebook/MICB323 Lab Book/2009/01/27: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 12: Line 12:
===Part 2: Transformation via electroporation using supplied p421 plasmid===
===Part 2: Transformation via electroporation using supplied p421 plasmid===
*Note: Sunny will do the p421 and CaCl<sub>2</sub>
*Note: Sunny will do the p421 and CaCl<sub>2</sub>
*
*Protocol followed as outlined in Lab Manual pages 37-38
*


===Part 3: Isolation of pure transformed colonies===
===Part 3: Isolation of pure transformed colonies===

Revision as of 16:19, 27 January 2009

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Lab 3: Plasmid Transformation of Competent Cells and Isolation of Transformants

Part 1: Transformation using ligation reaction and one shot INVaF' CaCl2 prepared competent cells

  • Protocol followed just as outlined in the Lab Manual pg 33

Part 2: Transformation via electroporation using supplied p421 plasmid

  • Note: Sunny will do the p421 and CaCl2
  • Protocol followed as outlined in Lab Manual pages 37-38

Part 3: Isolation of pure transformed colonies



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Eric_Ma/Notebook/MICB323_Lab_Book/2009/01/27&oldid=280365"