User:Eric Ma/Notebook/MICB323 Lab Book/2009/01/27: Difference between revisions

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{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Expt. Condition'''
| align="center" style="background:#f0f0f0;"|'''Expt. Condition'''
| align="center" style="background:#f0f0f0;"|'''Dilution Plated (10x)'''
| align="center" style="background:#f0f0f0;"|'''Dilution Plated (10<sup>x</sup>)'''
| align="center" style="background:#f0f0f0;"|'''Final Plated Volume (mL)'''
| align="center" style="background:#f0f0f0;"|'''Final Plated Volume (mL)'''
| align="center" style="background:#f0f0f0;"|'''Final Plated Dilution (10x)'''
| align="center" style="background:#f0f0f0;"|'''Final Plated Dilution (10<sup>x</sup>)'''
|-
|-
| Pre-Incubation Original Cells||-5||0.1||-6
| Pre-Incubation Original Cells||-5||0.1||-6

Revision as of 16:34, 27 January 2009

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Lab 3: Plasmid Transformation of Competent Cells and Isolation of Transformants

Part 1: Transformation using ligation reaction and one shot INVaF' CaCl2 prepared competent cells

  • Protocol followed just as outlined in the Lab Manual pg 33

Part 2: Transformation via electroporation using supplied p421 plasmid

  • Note: Sunny will do the p421 and CaCl2
  • Protocol followed as outlined in Lab Manual pages 37-38

Summary of Plates

Plates from Part 1

  • These are CaCl2 transformed cells using INVaF' competent cells and ligation reaction plasmid.
  • 3 plates:
Plated Volume (µL) Final Plated Dilution
200 0.2
100 0.1
50 0.05

Plates from Part 2

Expt. Condition Dilution Plated (10x) Final Plated Volume (mL) Final Plated Dilution (10x)
Pre-Incubation Original Cells -5 0.1 -6
Pre-Incubation Original Cells -6 0.1 -7
Transformed+DNA; LB-AMP + X-gal 0 0.1 -1
Transformed+DNA; LB-AMP + X-gal -1 0.1 -2
Transformed+DNA; LB-AMP + X-gal -2 0.1 -3
Transformed+DNA; LB -3 0.1 -4
Transformed+DNA; LB -4 0.1 -5
Transformed+DNA; LB -5 0.1 -6
\"Transformed\"+H2O; LB-AMP+X-gal 0 0.1 -1
\"Transformed\"+H2O; LB -3 0.1 -4
\"Transformed\"+H2O; LB -4 0.1 -5
\"Transformed\"+H2O; LB -5 0.1 -6
Post-Incubation Original Cells -6 0.1 -7
Post-Incubation Original Cells -7 0.1 -8


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