User:Eric Ma/Notebook/MICB323 Lab Book/2009/01/27: Difference between revisions
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==Summary of Plates== | ==Summary of Plates== | ||
===Dummy Demo Section=== | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Samples''' | |||
| align="center" style="background:#f0f0f0;"|'''Average Absorbance (A450)''' | |||
| align="center" style="background:#f0f0f0;"|'''100X Average Absorbance (A450)''' | |||
| align="center" style="background:#f0f0f0;"|'''Calculated [TNFa] (pg/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Normalized [TNFa] (pg/mL)''' | |||
|- | |||
| 0 hr PBS control||0.086||8.58||-342.36||0.00 | |||
|- | |||
| Day 1 +PBS control||0.109||10.85||-230.30||0.00 | |||
|- | |||
| 30 min +LPS||0.097||9.73||-285.71||0.00 | |||
|- | |||
| 60 min +LPS||0.102||10.20||-262.32||0.00 | |||
|- | |||
| 90 min +LPS||0.105||10.53||-246.31||0.00 | |||
|- | |||
| 6 hr +LPS||0.404||40.40||1225.37||1225.37 | |||
|- | |||
| 8 hr +LPS||0.131||13.08||-120.69||0.00 | |||
|- | |||
| Day 1 +LPS||0.227||22.65||1754.93||1754.93 | |||
|- | |||
| 90 min + IFNg||0.081||8.10||-365.76||0.00 | |||
|- | |||
| Day 1 +IFNg||0.103||10.33||-256.16||0.00 | |||
|- | |||
| 30 min +LPS +IFNg||0.086||8.55||-343.60||0.00 | |||
|- | |||
| 60 min +LPS +IFNg||0.093||9.33||-305.42||0.00 | |||
|- | |||
| 90 min +LPS +IFNg||0.099||9.93||-275.86||0.00 | |||
|- | |||
| 6 hr +LPS +IFNg||0.207||20.73||256.16||256.16 | |||
|- | |||
| 8 hr +LPS +IFNg||0.178||17.83||113.30||113.30 | |||
|- | |||
| Day 1 +LPS +IFNg||0.263||26.30||2653.94||2653.94 | |||
|} | |||
===Plates from Part 1=== | ===Plates from Part 1=== | ||
*These are CaCl<sub>2</sub> transformed cells using INVaF' competent cells and ligation reaction plasmid. | *These are CaCl<sub>2</sub> transformed cells using INVaF' competent cells and ligation reaction plasmid. |
Revision as of 15:59, 30 April 2009
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lab 3: Plasmid Transformation of Competent Cells and Isolation of TransformantsPart 1: Transformation using ligation reaction and one shot INVaF' CaCl2 prepared competent cells
Part 2: Transformation via electroporation using supplied p421 plasmid
Notes
Summary of PlatesDummy Demo Section
Plates from Part 1
Plates from Parts 2 and 3These plates come from electroporation using the p421 plasmid.
See this Excel spreadsheet to see how calculations were done. Summary
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