User:Eric Ma/Notebook/MICB323 Lab Book/2009/02/03: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2009/02/03 Entry for User:Eric_Ma/Notebook/MICB323_Lab_Book)
 
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Pre-Lab Calculations==
* Insert content here...
*Done as listed in this [[Media:090203_Manipulation_of_Restriction_Digest.xlsx|spreadsheet]].
 
*Volumes to be used:
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Variable'''
| align="center" style="background:#f0f0f0;"|'''Tube #'''
| align="center" style="background:#f0f0f0;"|'''pDNA (µL)'''
| align="center" style="background:#f0f0f0;"|'''EcoR1 (µL)'''
| align="center" style="background:#f0f0f0;"|'''10X React 3 (µL)'''
| align="center" style="background:#f0f0f0;"|'''500 mM NaCl (µL)'''
| align="center" style="background:#f0f0f0;"|'''500 mM Tris-HCl (µL)'''
| align="center" style="background:#f0f0f0;"|'''100 mM MgCl2 (µL)'''
| align="center" style="background:#f0f0f0;"|'''dH2O (µL)'''
| align="center" style="background:#f0f0f0;"|'''Total Volume (µL)'''
|-
| [enzyme]||1||0.2||0.5||2||0||0||0||17.3||20
|-
| [enzyme]||2||0.2||1||2||0||0||0||16.8||20
|-
| [enzyme]||3||0.2||1.5||2||0||0||0||16.3||20
|-
| [enzyme]||4||0.2||2||2||0||0||0||15.8||20
|-
| [NaCl]||1||0.2||0.5||2||1||0||0||16.3||20
|-
| [NaCl]||2||0.2||0.5||2||2||0||0||15.3||20
|-
| [NaCl]||3||0.2||0.5||2||3||0||0||14.3||20
|-
| [NaCl]||4||0.2||0.5||2||4||0||0||13.3||20
|}
*Calculation table:
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Component'''
| align="center" style="background:#f0f0f0;"|'''Tube'''
| align="center" style="background:#f0f0f0;"|'''[NaCl] in 10X React 3 (mM)'''
| align="center" style="background:#f0f0f0;"|'''[NaCl] Provided (mM)'''
| align="center" style="background:#f0f0f0;"|'''[NaCl] Wanted (10X) (mM)'''
| align="center" style="background:#f0f0f0;"|'''Extra [NaCl] needed (mM)'''
| align="center" style="background:#f0f0f0;"|'''500 mM NaCl (µL)'''
|-
| NaCl||1||500||500||1000||500||1
|-
| NaCl||2||500||500||1500||1000||2
|-
| NaCl||3||500||500||2000||1500||3
|-
| NaCl||4||500||500||2500||2000||4
|}


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 11:36, 3 February 2009

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Pre-Lab Calculations

  • Done as listed in this spreadsheet.
  • Volumes to be used:
Variable Tube # pDNA (µL) EcoR1 (µL) 10X React 3 (µL) 500 mM NaCl (µL) 500 mM Tris-HCl (µL) 100 mM MgCl2 (µL) dH2O (µL) Total Volume (µL)
[enzyme] 1 0.2 0.5 2 0 0 0 17.3 20
[enzyme] 2 0.2 1 2 0 0 0 16.8 20
[enzyme] 3 0.2 1.5 2 0 0 0 16.3 20
[enzyme] 4 0.2 2 2 0 0 0 15.8 20
[NaCl] 1 0.2 0.5 2 1 0 0 16.3 20
[NaCl] 2 0.2 0.5 2 2 0 0 15.3 20
[NaCl] 3 0.2 0.5 2 3 0 0 14.3 20
[NaCl] 4 0.2 0.5 2 4 0 0 13.3 20
  • Calculation table:
Component Tube [NaCl] in 10X React 3 (mM) [NaCl] Provided (mM) [NaCl] Wanted (10X) (mM) Extra [NaCl] needed (mM) 500 mM NaCl (µL)
NaCl 1 500 500 1000 500 1
NaCl 2 500 500 1500 1000 2
NaCl 3 500 500 2000 1500 3
NaCl 4 500 500 2500 2000 4


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Eric_Ma/Notebook/MICB323_Lab_Book/2009/02/03&oldid=282328"