User:Eric Ma/Notebook/MICB323 Lab Book/2009/02/03: Difference between revisions
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==Part 1: Plasmid Extraction== | ==Part 1: Plasmid Extraction== | ||
* Electroporation transformed: No growth. | * Electroporation transformed: No growth. | ||
* CaCl<sub>2</sub> untransformed: Growth. | * EM#1 - CaCl<sub>2</sub> untransformed: Growth. | ||
* CaCl<sub>2</sub> transformed: Growth. | * EM#2 - CaCl<sub>2</sub> transformed: Growth. | ||
* I will use Sunny's culture's DNA. | * I will use Sunny's culture's DNA. | ||
<br> | <br> | ||
*Entire ON culture - spin @8000rpm @3min. Performed split up into two spins in same tubes. | *Entire ON culture - spin @8000rpm @3min. Performed split up into two spins in same tubes. | ||
**Note: Found out that not all of the EM#2 was transferred into the 2nd spin. There will be 750µL less (i.e. 1/4 less) cells present. This may reduce [pDNA] obtained in the end; expected to be 1/4 less compared to EM#1. | |||
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Revision as of 15:08, 3 February 2009
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Pre-Lab Calculations
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Part 1: Plasmid Extraction
- Electroporation transformed: No growth.
- EM#1 - CaCl2 untransformed: Growth.
- EM#2 - CaCl2 transformed: Growth.
- I will use Sunny's culture's DNA.
- Entire ON culture - spin @8000rpm @3min. Performed split up into two spins in same tubes.
- Note: Found out that not all of the EM#2 was transferred into the 2nd spin. There will be 750µL less (i.e. 1/4 less) cells present. This may reduce [pDNA] obtained in the end; expected to be 1/4 less compared to EM#1.