User:Eric Ma/Notebook/MICB323 Lab Book/2009/02/10: Difference between revisions
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# Crystal and Mark did the spinning + extraction of lysates. | # Crystal and Mark did the spinning + extraction of lysates. | ||
# Sunny and I will do the recombinant virus samples; Mark and Crystal will do the wild-type virus samples. | # Sunny and I will do the recombinant virus samples; Mark and Crystal will do the wild-type virus samples. | ||
## Sunny does BCA assay | ## Sunny does BCA assay. <b>(This did not happen.)</b> | ||
## I do BSA assay. | ## I do BSA assay. <b>(This did not happen. See below)</b> | ||
===Problem: SDS Added to Recombinant Viral Lysates=== | ===Problem: SDS Added to Recombinant Viral Lysates=== |
Revision as of 17:39, 1 March 2009
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Part 1: Agarose Gel of Plasmid DNA from Previous Lab
Summary of Tube Labels
Lanes on the gel and volumes added to the wells
Gel was run for 1 hour @ 120 volts. Noticed that the samples in lanes 3, 5 and 7 had a tendency to float off. Part 3: Determination of Viral Lysate Protein Concentrations using the Bradford and Bicinchoninic Acid Protein AssaysNotes
Problem: SDS Added to Recombinant Viral Lysates
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