User:Eric Ma/Notebook/MICB323 Lab Book/2009/02/10: Difference between revisions
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==Results== | ==Results== | ||
===BCA Assay Results=== | ===BCA Assay Plate Results=== | ||
* Eric's Plate <b>(Need to check whether this was recombinant or WT virus)</b> | * Eric's Plate <b>(Need to check whether this was recombinant or WT virus)</b> | ||
{| {{table}} | {| {{table}} | ||
Line 90: | Line 90: | ||
| H||0.703||0.686||0.723||0.754||0.754||0.778||0.034||0.034||0.034||0.035||0.034||0.034 | | H||0.703||0.686||0.723||0.754||0.754||0.778||0.034||0.034||0.034||0.035||0.034||0.034 | ||
|} | |} | ||
* Guide to plate: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Wells''' | |||
| align="center" style="background:#f0f0f0;"|'''Sample''' | |||
|- | |||
| A1-A6||0 µg/mL | |||
|- | |||
| B1-B6||25 µg/mL | |||
|- | |||
| C1-C6||125 µg/mL | |||
|- | |||
| D1-D6||250 µg/mL | |||
|- | |||
| E1-E6||500 µg/mL | |||
|- | |||
| F1-F6||750 µg/mL | |||
|- | |||
| G1-G6||1000 µg/mL | |||
|- | |||
| H1-H6||1500 µg/mL | |||
|- | |||
| A7-A12||2000 µg/mL | |||
|- | |||
| B7-B12||2.5 hr (-) virus | |||
|- | |||
| C7-C12||2.5 hr (+) virus | |||
|- | |||
| D7-D12||2nd harvest + virus | |||
|- | |||
| E7-E12||3rd harvest + virus | |||
|- | |||
| F7-F12||4th harvest + virus | |||
|- | |||
| G7-G12||5th harvest + virus | |||
|- | |||
| H7-H12||N/A | |||
|} | |||
* Sunny's Plate <b>(Need to check whether this was recombinant or WT virus)</b> | * Sunny's Plate <b>(Need to check whether this was recombinant or WT virus)</b> | ||
{| {{table}} | {| {{table}} | ||
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|- | |- | ||
| H||0.684||0.708||0.622||0.591||0.609||0.642||0.415||0.325||0.265||0.267||0.034||0.034 | | H||0.684||0.708||0.622||0.591||0.609||0.642||0.415||0.325||0.265||0.267||0.034||0.034 | ||
|} | |||
* Guide to plate: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Wells''' | |||
| align="center" style="background:#f0f0f0;"|'''Sample''' | |||
|- | |||
| A1-A6||0 µg/mL | |||
|- | |||
| B1-B6||25 µg/mL | |||
|- | |||
| C1-C6||125 µg/mL | |||
|- | |||
| D1-D6||250 µg/mL | |||
|- | |||
| E1-E6||500 µg/mL | |||
|- | |||
| F1-F6||750 µg/mL | |||
|- | |||
| G1-G6||1000 µg/mL | |||
|- | |||
| H1-H6||1500 µg/mL | |||
|- | |||
| A7-A12||2000 µg/mL | |||
|- | |||
| B7-B12||N/A | |||
|- | |||
| C7-C12||2.5 hr (-) virus | |||
|- | |||
| D7-D12||2.5 hr (+) virus | |||
|- | |||
| E7-E12||2nd harvest + virus | |||
|- | |||
| F7-F12||3rd harvest + virus | |||
|- | |||
| G7-G12||4th harvest + virus | |||
|- | |||
| H7-H12||5th harvest + virus | |||
|} | |} | ||
Revision as of 20:54, 1 March 2009
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Part 1: Agarose Gel of Plasmid DNA from Previous Lab
Summary of Tube Labels
Lanes on the gel and volumes added to the wells
Gel was run for 1 hour @ 120 volts. Noticed that the samples in lanes 3, 5 and 7 had a tendency to float off. Part 3: Determination of Viral Lysate Protein Concentrations using the Bradford and Bicinchoninic Acid Protein AssaysNotes
Problem: SDS Added to Recombinant Viral Lysates
ResultsBCA Assay Plate Results
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