User:Eric Ma/Notebook/MICB323 Lab Book/2009/02/10: Difference between revisions
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# EM2 - Transformed and digested pDNA from CaCl<sub>2</sub> (10 µL Sample + 2µL 6X Loading Buffer) | # EM2 - Transformed and digested pDNA from CaCl<sub>2</sub> (10 µL Sample + 2µL 6X Loading Buffer) | ||
# EM#2 - Isolated Transformed pDNA from CaCl<sub>2</sub> (10 µL Sample + 2µL 6X Loading Buffer) | # EM#2 - Isolated Transformed pDNA from CaCl<sub>2</sub> (10 µL Sample + 2µL 6X Loading Buffer) | ||
# EM3 - | # EM3 - Digested p421 plasmid DNA inserted via CaCl<sub>2</sub> (10 µL Sample + 2µL 6X Loading Buffer) (Note: From Sunny) | ||
# EM#3 - | # EM#3 - Undigested p421 plasmid DNA inserted via CaCl<sub>2</sub> (10 µL Sample + 2µL 6X Loading Buffer) (Note: From Sunny) | ||
# Ligation Reaction (8µL including 1.5µL of 6X Loading Buffer) | # Ligation Reaction (8µL including 1.5µL of 6X Loading Buffer) | ||
# Mass Ruler (10 µL) | # Mass Ruler (10 µL) |
Revision as of 18:30, 8 March 2009
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Part 1: Agarose Gel of Plasmid DNA from Previous Lab
Summary of Tube Labels
Lanes on the gel and volumes added to the wells
Gel was run for 1 hour @ 120 volts. Noticed that the samples in lanes 3, 5 and 7 had a tendency to float off. Part 3: Determination of Viral Lysate Protein Concentrations using the Bradford and Bicinchoninic Acid Protein AssaysNotes
Problem: SDS Added to Recombinant Viral Lysates
ResultsGel ElectrophoresisBCA Assay Plate Results
Eric's Plate (Need to check whether this was recombinant or WT virus)
Sunny's Plate (Need to check whether this was recombinant or WT virus)
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