User:Eric Ma/Notebook/MICB323 Lab Book/2009/02/10: Difference between revisions
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===Gel Electrophoresis=== | ===Gel Electrophoresis=== | ||
[[Image:090210_Gel_eric_ma.tif|800px]] | [[Image:090210_Gel_eric_ma.tif|800px]] | ||
===Southern Hybridization=== | |||
[[Image:090303_Southern_Image.tif|800px]] | [[Image:090303_Southern_Image.tif|800px]] | ||
Revision as of 13:19, 9 March 2009
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Part 1: Agarose Gel of Plasmid DNA from Previous Lab
Summary of Tube Labels
Lanes on the gel and volumes added to the wells
Gel was run for 1 hour @ 120 volts. Noticed that the samples in lanes 3, 5 and 7 had a tendency to float off. Part 3: Determination of Viral Lysate Protein Concentrations using the Bradford and Bicinchoninic Acid Protein AssaysNotes
Problem: SDS Added to Recombinant Viral Lysates
ResultsGel ElectrophoresisSouthern HybridizationBCA Assay Plate Results
Eric's Plate (Need to check whether this was recombinant or WT virus)
Sunny's Plate (Need to check whether this was recombinant or WT virus)
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