User:Eric Ma/Notebook/MICB323 Lab Book/2009/02/10: Difference between revisions
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| 5th harvest + virus||516.1 | | 5th harvest + virus||516.1 | ||
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===BSA Assay Results=== | |||
*Notes: Done up by Crystal | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Tube''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration of BSA (mg/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Vol BSA''' | |||
| align="center" style="background:#f0f0f0;"|'''Vol H2O''' | |||
| align="center" style="background:#f0f0f0;"|'''Vol Bradford''' | |||
| align="center" style="background:#f0f0f0;"|'''Abs value''' | |||
| align="center" style="background:#f0f0f0;"|'''100X Abs''' | |||
| align="center" style="background:#f0f0f0;"|'''Calc. Conc (mg/mL)''' | |||
|- | |||
| 1||0||0||100||3||0||0||-0.00112435 | |||
|- | |||
| 2||0.1||10||90||3||0.101||10.1||0.152958855 | |||
|- | |||
| 3||0.15||15||85||3||0.109||10.9||0.165163465 | |||
|- | |||
| 4||0.2||20||80||3||0.117||11.7||0.177368076 | |||
|- | |||
| 5||0.25||25||75||3||0.128||12.8||0.194149415 | |||
|- | |||
| 6||0.3||30||70||3||0.232||23.2||0.352809349 | |||
|- | |||
| 7||0.35||35||65||3||0.232||23.2||0.352809349 | |||
|- | |||
| 8||0.4||40||60||3||0.304||30.4||0.462650841 | |||
|- | |||
| 9||2.5 hr (-)||10||90||3||0.128||12.8||0.194149415 | |||
|- | |||
| 10||2.5 hr (+)||10||90||3||0.178||17.8||0.270428229 | |||
|- | |||
| 11||2nd H (+)||10||90||3||0.209||20.9||0.317721094 | |||
|- | |||
| 12||3rd H (+)||10||90||3||0.258||25.8||0.392474332 | |||
|- | |||
| 13||4th H (+)||10||90||3||0.284||28.4||0.432139316 | |||
|- | |||
| 14||5th H (+)||10||90||3||0.319||31.9||0.485534486 | |||
|} | |||
[[Image:BSA_Standard_Curve.png|800px]] | |||
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Revision as of 13:39, 10 March 2009
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Part 1: Agarose Gel of Plasmid DNA from Previous Lab
Summary of Tube Labels
Lanes on the gel and volumes added to the wells
Gel was run for 1 hour @ 120 volts. Noticed that the samples in lanes 3, 5 and 7 had a tendency to float off. Part 3: Determination of Viral Lysate Protein Concentrations using the Bradford and Bicinchoninic Acid Protein AssaysNotes
Problem: SDS Added to Recombinant Viral Lysates
ResultsGel ElectrophoresisSouthern HybridizationBCA Assay Plate Results
Eric's Plate (Recombinant Virus)
Sunny's Plate (Wild-Type Virus)
BSA Assay Results
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