User:Eric Ma/Notebook/MICB323 Lab Book/2009/03/10: Difference between revisions
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{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Sample''' | | align="center" style="background:#f0f0f0;"|'''Sample''' | ||
| align="center" style="background:#f0f0f0;"|'''Concentration | | align="center" style="background:#f0f0f0;"|'''Protein Concentration in Original Sample (mg/mL)''' | ||
| align="center" style="background:#f0f0f0;"|'''Volume Needed for SDS-PAGE''' | | align="center" style="background:#f0f0f0;"|'''Volume Needed for SDS-PAGE (µL)''' | ||
|- | |- | ||
| WT 2.5 hr (-)||0.194||15 | | WT 2.5 hr (-)||0.194||15 | ||
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|} | |} | ||
* Calculations for BCA Assay using | * Calculations for BCA Assay using WT Virus: | ||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Sample''' | | align="center" style="background:#f0f0f0;"|'''Sample''' | ||
| align="center" style="background:#f0f0f0;"|'''Concentration | | align="center" style="background:#f0f0f0;"|'''Protein Concentration in Original Sample (µg/mL)''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''Volume Needed for SDS-PAGE (µL)''' | ||
|- | |- | ||
| 2.5 hr (-) virus||1225.8||15.00 | | WT 2.5 hr (-) virus||1225.8||15.00 | ||
|- | |- | ||
| 2.5 hr (+) virus||1627.6||11.30 | | WT 2.5 hr (+) virus||1627.6||11.30 | ||
|- | |- | ||
| 2nd harvest + virus||4448.7||4.13 | | WT 2nd harvest + virus||4448.7||4.13 | ||
|- | |- | ||
| 3rd harvest + virus||2750.7||6.68 | | WT 3rd harvest + virus||2750.7||6.68 | ||
|- | |- | ||
| 4th harvest + virus||3926.7||4.68 | | WT 4th harvest + virus||3926.7||4.68 | ||
|- | |- | ||
| 5th harvest + virus||2064.5||8.91 | | WT 5th harvest + virus||2064.5||8.91 | ||
|} | |} | ||
Revision as of 16:01, 10 March 2009
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Pre-Lab Calculations for SDS-PAGE
Part 1: Determination of Protein Concentration of LPS-Stimulated Cel Lysates using Bradford Assay
Part 2: SDS-PAGE of Insect Viral Lysates
ResultsPart 1: BSA Assay
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