User:Eric Ma/Notebook/MICB323 Lab Book/2009/03/24: Difference between revisions
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| H||4000 pg/mL TNFa||90 min + IFNg||Day 1 +IFNg | | H||4000 pg/mL TNFa||90 min + IFNg||Day 1 +IFNg | ||
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==Part 2: SDS-PAGE== | |||
Wells loaded as follows. | |||
* Notes: | |||
** The gel ran in a funny manner - it was uneven, and resulted in slanting. As the pattern was identical on both my plate and Sunny's plate (i.e. ours were mirror images of each other), we think it may be due to a voltage problem. The wiring may be causing this. | |||
** As a result, it will be hard for us to measure any sizes of the proteins. | |||
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Revision as of 15:02, 24 March 2009
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Goals
Part 1: ELISA Detection of TNFa in LPS +/- IFNg stimulated culture supernatants.
Part 2: SDS-PAGEWells loaded as follows.
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