User:Eric Ma/Notebook/MICB323 Lab Book/2009/03/24: Difference between revisions
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* Plate incubation time after addition of TMB: 15 minutes | * Plate incubation time after addition of TMB: 15 minutes | ||
** Hopefully there is enough time for color formation. | ** Hopefully there is enough time for color formation. | ||
** As it turns out, there wasn't a high enough concentration of streptavidin-HRP. Jen has gone to prepare more strep-HRP, which we will add 50µL more of the concentrated strep-HRP. This way, we hope to get more color production faster. | |||
==Part 2: SDS-PAGE== | ==Part 2: SDS-PAGE== |
Revision as of 16:46, 24 March 2009
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Goals
Part 1: ELISA Detection of TNFa in LPS +/- IFNg stimulated culture supernatants.96 Well Immunoplate Layout
Notes
Part 2: SDS-PAGEEric's Gel
Sunny's Gel
Notes
Part 3: Western TransferNotes
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