User:Eric Ma/Notebook/MICB323 Lab Book/2009/03/24: Difference between revisions
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| align="center" style="background:#f0f0f0;"|'''Samples''' | | align="center" style="background:#f0f0f0;"|'''Samples''' | ||
| align="center" style="background:#f0f0f0;"|'''Average Absorbance (A<sub>450</sub>)''' | | align="center" style="background:#f0f0f0;"|'''Average Absorbance (A<sub>450</sub>)''' | ||
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| 0 pg TNFa (assay buffer only)||0.12875 | |||
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| 62.5 pg/mL TNFa||0.141 | |||
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| 125 pg/mL TNFa||0.1775 | |||
|- | |||
| 250 pg/mL TNFa||0.2105 | |||
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| 500 pg/mL TNFa||0.272 | |||
|- | |||
| 1000 pg/mL TNFa||0.40925 | |||
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| 2000 pg/mL TNFa||0.324 | |||
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| 4000 pg/mL TNFa||0.95225 | |||
|- | |- | ||
| 0 hr PBS control||0.08575 | | 0 hr PBS control||0.08575 |
Revision as of 22:36, 31 March 2009
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Goals
Part 1: ELISA Detection of TNFa in LPS +/- IFNg stimulated culture supernatants.96 Well Immunoplate Layout
Notes
Part 2: SDS-PAGEEric's Gel
Sunny's Gel
Notes
Part 3: Western TransferNotes
ResultsELISA Results
Tabluated Average Absorbance Data
Standard Curve |