View source for User:Eric Ma/Notebook/iGEM 2010 Lab Prep/2010/05/11

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==Test of old BW27783 + P<sub>BAD</sub>-RBS-RFP-Term Cultures==
* Found an old plate of BW27783s containing P<sub>BAD</sub>-RBS-RFP-Terminator in the 4ºC walk-in room
* Tested by streaking on an Amp-100 plate + 0.1% (w/v) arabinose. Control: No arabinose
* No growth observed on the next day.

==Test of Old Antibiotic Stocks==
* Tested Amp100, Kan50 and Chlor25 from the old batch of antibiotics in the -20ºC freezer.
* 3 ml LB + 3 µl antibiotics (each)
* Inoculated using DH5α competent cells. 
* Results:
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Amp100'''
| align="center" style="background:#f0f0f0;"|'''Kan50'''
| align="center" style="background:#f0f0f0;"|'''Chlor25'''
| align="center" style="background:#f0f0f0;"|'''LB-only'''
|-
| No growth||No growth||No growth||Growth
|}
* This is a good sign. Antibiotic batches are still working.


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