User:Eric Ma/Notebook/iGEM 2010 Lab Prep/2010/05/11

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Test of old BW27783 + PBAD-RBS-RFP-Term Cultures

  • Found an old plate of BW27783s containing PBAD-RBS-RFP-Terminator in the 4ºC walk-in room
  • Tested by streaking on an Amp-100 plate + 0.1% (w/v) arabinose. Control: No arabinose
  • No growth observed on the next day.

Test of Old Antibiotic Stocks

  • Tested Amp100, Kan50 and Chlor25 from the old batch of antibiotics in the -20ºC freezer.
  • 3 ml LB + 3 µl antibiotics (each)
  • Inoculated using DH5α competent cells.
  • Results:
Amp100 Kan50 Chlor25 LB-only
No growth No growth No growth Growth
  • This is a good sign. Antibiotic batches are still working.



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