User:Eric Ma/Notebook/iGEM 2010 Lab Prep/2010/05/12

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Test of PCR Reagents)
(Test of Miniprep Reagents)
Line 9: Line 9:
* From the cultures that are currently growing, I will do a test of the miniprep reagents leftover from last year.  
* From the cultures that are currently growing, I will do a test of the miniprep reagents leftover from last year.  
* Buffer P1 is in the 4ºC fridge, P2, Neutralization etc. are on Tony Lam's bench right now.
* Buffer P1 is in the 4ºC fridge, P2, Neutralization etc. are on Tony Lam's bench right now.
-
* Will do this in the afternoon, after JC meeting.
+
* Ran miniprep with iGEMers in the afternoon:
 +
** Each person took 1.5 mL of culture from one of the three tubes, and spun down according to Rafael's protocol.
 +
** Ignored the 5 min. waiting step after adding in lysis buffer.
 +
** Stored at 4ºC overnight.
 +
* Results:
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Sample'''
 +
| align="center" style="background:#f0f0f0;"|'''[DNA] (ng/µl)'''
 +
| align="center" style="background:#f0f0f0;"|'''A<sub>260</sub>/A<sub>280</sub>'''
 +
| align="center" style="background:#f0f0f0;"|'''Notes'''
 +
|-
 +
| EF||92.9||2.02||
 +
|-
 +
| MW||70.6||2.06||Dried using spinning vac
 +
|-
 +
| JG||182.7||2.05||
 +
|-
 +
| PC||56.7||1.99||
 +
|-
 +
| VM||98.0||1.94||
 +
|}
 +
* Some notes about this:
 +
*# The samples were miniprepped using cultures that were not very dense (didn't measure OD, so not very sure on this), but they certainly didn't look like they had reached stat phase in growth. This might explain the small amount of DNA present.

Revision as of 16:01, 13 May 2010

Project name Main project page
Previous entry      Next entry

Test of Miniprep Reagents

  • From the cultures that are currently growing, I will do a test of the miniprep reagents leftover from last year.
  • Buffer P1 is in the 4ºC fridge, P2, Neutralization etc. are on Tony Lam's bench right now.
  • Ran miniprep with iGEMers in the afternoon:
    • Each person took 1.5 mL of culture from one of the three tubes, and spun down according to Rafael's protocol.
    • Ignored the 5 min. waiting step after adding in lysis buffer.
    • Stored at 4ºC overnight.
  • Results:
Sample [DNA] (ng/µl) A260/A280 Notes
EF92.92.02
MW70.62.06Dried using spinning vac
JG182.72.05
PC56.71.99
VM98.01.94
  • Some notes about this:
    1. The samples were miniprepped using cultures that were not very dense (didn't measure OD, so not very sure on this), but they certainly didn't look like they had reached stat phase in growth. This might explain the small amount of DNA present.



Personal tools