User:Eric Ma/Notebook/iGEM 2010 Lab Prep/2010/05/13: Difference between revisions
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| 10X Buffer 2||2||2||2||2||2||2||2||2||2||20 | | 10X Buffer 2||2||2||2||2||2||2||2||2||2||20 | ||
|- | |- | ||
| EcoRI||0||1||0||0||0||1||1||0||0|| | | EcoRI||0||1||0||0||0||1||1||0||0||N/A | ||
|- | |- | ||
| XbaI||0||0||1||0||0||0||0||1||1|| | | XbaI||0||0||1||0||0||0||0||1||1||N/A | ||
|- | |- | ||
| SpeI||0||0||0||1||0||1||0||1||0|| | | SpeI||0||0||0||1||0||1||0||1||0||N/A | ||
|- | |- | ||
| PstI||0||0||0||0||1||0||1||0||1|| | | PstI||0||0||0||0||1||0||1||0||1||N/A | ||
|- | |- | ||
| DNA||3||3||3||3||3||3||3||3||3||30 | | DNA||3||3||3||3||3||3||3||3||3||30 | ||
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* Digest for ~1-2 hrs (not so crucial) at room temp. | * Digest for ~1-2 hrs (not so crucial) at room temp. | ||
* Note: Initially wanted only 10 µl of water, but in the end, I calculated for 30 µl instead in the master mix for a total of 300 µl of water. Therefore, the reaction may be a bit dilute, which is why I will let this run while I continue writing and rehearsing the podcast instead. | * Note: Initially wanted only 10 µl of water, but in the end, I calculated for 30 µl instead in the master mix for a total of 300 µl of water. Therefore, the reaction may be a bit dilute, which is why I will let this run while I continue writing and rehearsing the podcast instead. The timing shouldn't be too important. | ||
* Digest ran overnight on ice after ~2h incubation in 37ºC. | |||
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Revision as of 10:30, 14 May 2010
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Restriction Enzyme Test
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