User:Etchevers/Notebook/Conference notes/2009/03/26: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 23: Line 23:
*'''[[User:Etchevers|Heather]] 05:25, 26 March 2009 (EDT)''':
*'''[[User:Etchevers|Heather]] 05:25, 26 March 2009 (EDT)''':


Finally finished them all - mapping to sequence, highlighting, and making chart with suggested amplification temperatures and amplicon sizes.
*'''[[User:Etchevers|Heather]] 10:10, 26 March 2009 (EDT)''':


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 07:10, 26 March 2009

Conference and seminar notes <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Notes for primer design for candidate gene sequencing

With Tania, choosing primers to test in lethal fetal pathology. First gene is 36 exons, designed 31 amplicons but must keep the following things in mind! Now need to redesign most of them. Also for another gene, 6 amplicons; total of 48 made yesterday and remaining 11 to redo.

1. Choose preferentially around 60°C Tm

2. Maximum comfortable length 600 bp

3. At least 50 bp flanking each for two reasons:

  • sequencing always swallows up first nucleotides with blobs of dye and so forth.
  • Heather 05:25, 26 March 2009 (EDT):

Finally finished them all - mapping to sequence, highlighting, and making chart with suggested amplification temperatures and amplicon sizes.

  • Heather 10:10, 26 March 2009 (EDT):


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Etchevers/Notebook/Conference_notes/2009/03/26&oldid=296903"