User:Etienne Robillard/Notebook/30C6HSL - Revision history

Etienne Robillard: /* Light-activated morphogenesis of breast cancer through activation of the "S1PR4" receptor */

Etienne Robillard — Sun, 28 Apr 2013 15:57:38 GMT

Light-activated morphogenesis of breast cancer through activation of the "S1PR4" receptor

←Older revision		Revision as of 15:57, 28 April 2013
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	** http://www.uniprot.org/uniprot/P24182		** http://www.uniprot.org/uniprot/P24182
	** http://www.uniprot.org/uniprot/Q13094		** http://www.uniprot.org/uniprot/Q13094
-	=== Light-activated morphogenesis of breast cancer through activation of the "S1PR4~~" receptor: Unexpected findings from the synthesis of benzoxazole derivatives raise new questions on the risks of orthogonal riboswitch design~~ ===	+	=== Light-activated morphogenesis of breast cancer through exogenous activation of the S1PR4 (erbb2) signaling pathway ===
	** http://www.ncbi.nlm.nih.gov/nuccore/NM_003775.3 ''"GeneRIF: findings highlight an important role for S1P(4) and SK1 in ER(-) breast cancer progression."''		** http://www.ncbi.nlm.nih.gov/nuccore/NM_003775.3 ''"GeneRIF: findings highlight an important role for S1P(4) and SK1 in ER(-) breast cancer progression."''
	** ''"Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer."'' http://www.ncbi.nlm.nih.gov/protein/NP_003766.1		** ''"Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer."'' http://www.ncbi.nlm.nih.gov/protein/NP_003766.1
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	** http://omim.org/entry/603751		** http://omim.org/entry/603751
	** http://omim.org/entry/601974		** http://omim.org/entry/601974
-	** google key words: ~~benzoxazole~~, CAMK2B, S1PR4 ~~(Breast cancer)~~	+	** Get the Free PMC [http://www.ncbi.nlm.nih.gov/pubmed/20837468 here].
		+	** http://www.kegg.jp/dbget-bin/www_bget?K06279
		+	** google key words: benzoxadiazole, CAMK2B, erbb2, S1PR4, MAPK, SHC
	** Horn-directed "S1PR4" mutant protein amplification and overexpression (Calmodulin/Rhodopsin-dependent orthogonal riboswitch): http://www.uniprot.org/uniprot/O95977		** Horn-directed "S1PR4" mutant protein amplification and overexpression (Calmodulin/Rhodopsin-dependent orthogonal riboswitch): http://www.uniprot.org/uniprot/O95977

Etienne Robillard at 19:05, 14 April 2013

Etienne Robillard — Sun, 14 Apr 2013 19:05:22 GMT

←Older revision		Revision as of 19:05, 14 April 2013
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	#ref2 pmid=11248055		#ref2 pmid=11248055
	</biblio>		</biblio>
		+
		+	=== Did you know? ===
		+	<blockquote>
		+	“The democracy will cease to exist when you take away from those who are willing to work and give to those who would not.” – Thomas Jefferson
		+	</blockquote>

Etienne Robillard: /* Light (round the horn) directed S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12) */

Etienne Robillard — Sat, 13 Apr 2013 23:18:49 GMT

Light (round the horn) directed S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12)

←Older revision		Revision as of 23:18, 13 April 2013
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	=== Light (round the horn) directed S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12) ===		=== Light (round the horn) directed S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12) ===
	* Breast cancer/Alzheimer/Leukemia (DS, Parkinson, etc): Rhodopsin (Rho) gene-level interactions with <font color=red>S1PR4-directed</font> protein overexpression lineage in HEK293 important note: <font color="red">''The Rho signalling pathway is activated, for example, by bioactive sphingolipids such as sphingosine-1-phosphate (SPP) or by overexpression of Rho family members in tumorigenesis and metastases [16].'' </font> [Note: see [[User:Etienne_Robillard/Notebook/Rhodopsin\|Rhodopsin]] for info on photophosphorylation of the Rhodopsin kinase, a <font color=purple>Ca2-dependent photoreceptor/GPCR transcription pathway?</font>]		* Breast cancer/Alzheimer/Leukemia (DS, Parkinson, etc): Rhodopsin (Rho) gene-level interactions with <font color=red>S1PR4-directed</font> protein overexpression lineage in HEK293 important note: <font color="red">''The Rho signalling pathway is activated, for example, by bioactive sphingolipids such as sphingosine-1-phosphate (SPP) or by overexpression of Rho family members in tumorigenesis and metastases [16].'' </font> [Note: see [[User:Etienne_Robillard/Notebook/Rhodopsin\|Rhodopsin]] for info on photophosphorylation of the Rhodopsin kinase, a <font color=purple>Ca2-dependent photoreceptor/GPCR transcription pathway?</font>]
-	* Questions/Comments:
-	# Which light-activated (AHL) synthetic transcription promoters the DNA engineers may have chosen to induce Calmodulin-dependent '''psychotic''' behavior and <font color=blue>visual hallucinations</font> as the primary phenotype? -[[User:Etienne_Robillard\|ER]]
-	~~# What is the technique (protocol name) name the "biological programmers" may have implemented to induce synthetic neurological circuits or programs? -[[User:Etienne_Robillard\|ER]]~~
-	# Based on your research, what is a neuronal aptamer primary biological functions and describe the key concepts and techniques in the "hijacking" of human DNA as a information storage transcriptional '''vector''' -[[User:Etienne_Robillard\|ER]]
	* Notes:		* Notes:
	# To obtain the consensus pattern, see Accession number PS00237 [http://prosite.expasy.org/PS00237 here]. ([http://prosite.expasy.org/cgi-bin/aligner?psa=PS00237&format=FASTA FASTA]).		# To obtain the consensus pattern, see Accession number PS00237 [http://prosite.expasy.org/PS00237 here]. ([http://prosite.expasy.org/cgi-bin/aligner?psa=PS00237&format=FASTA FASTA]).

Etienne Robillard: /* Light (round the horn) directed S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12) */

Etienne Robillard — Sat, 13 Apr 2013 22:33:13 GMT

Light (round the horn) directed S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12)

←Older revision		Revision as of 22:33, 13 April 2013
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	=== Light (round the horn) directed S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12) ===		=== Light (round the horn) directed S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12) ===
-	* Breast cancer/Alzheimer/Leukemia (DS, Parkinson, etc): Rhodopsin (Rho) gene-level interactions with <font color=red>S1PR4-directed</font> protein overexpression lineage in HEK293 important note: <font color="red">''The Rho signalling pathway is activated, for example, by bioactive sphingolipids such as sphingosine-1-phosphate (SPP) or by overexpression of Rho family members in tumorigenesis and metastases [16].'' </font> [Note: see [[User:Etienne_Robillard/Notebook/Rhodopsin\|Rhodopsin]] for info on photophosphorylation of the Rhodopsin kinase, a Ca2-dependent photoreceptor/GPCR]	+	* Breast cancer/Alzheimer/Leukemia (DS, Parkinson, etc): Rhodopsin (Rho) gene-level interactions with <font color=red>S1PR4-directed</font> protein overexpression lineage in HEK293 important note: <font color="red">''The Rho signalling pathway is activated, for example, by bioactive sphingolipids such as sphingosine-1-phosphate (SPP) or by overexpression of Rho family members in tumorigenesis and metastases [16].'' </font> [Note: see [[User:Etienne_Robillard/Notebook/Rhodopsin\|Rhodopsin]] for info on photophosphorylation of the Rhodopsin kinase, a <font color=purple>Ca2-dependent photoreceptor/GPCR transcription pathway?</font>]
	* Questions/Comments:		* Questions/Comments:
	# Which light-activated (AHL) synthetic transcription promoters the DNA engineers may have chosen to induce Calmodulin-dependent '''psychotic''' behavior and <font color=blue>visual hallucinations</font> as the primary phenotype? -[[User:Etienne_Robillard\|ER]]		# Which light-activated (AHL) synthetic transcription promoters the DNA engineers may have chosen to induce Calmodulin-dependent '''psychotic''' behavior and <font color=blue>visual hallucinations</font> as the primary phenotype? -[[User:Etienne_Robillard\|ER]]

Etienne Robillard: /* Design of your mutant g-protein receptor: From 30C6HSL to Heterocyclics oxazole */

Etienne Robillard — Sat, 13 Apr 2013 21:14:45 GMT

Design of your mutant g-protein receptor: From 30C6HSL to Heterocyclics oxazole

←Older revision		Revision as of 21:14, 13 April 2013
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	** http://www.uniprot.org/uniprot/P24182		** http://www.uniprot.org/uniprot/P24182
	** http://www.uniprot.org/uniprot/Q13094		** http://www.uniprot.org/uniprot/Q13094
-	* <font color=~~"red">Morphology~~ of breast cancer through activation of the "S1PR4" receptor~~, an unexpected finding~~ from the synthesis of ~~oxazole~~ derivatives~~</font>:~~	+	=== Light-activated morphogenesis of breast cancer through activation of the "S1PR4" receptor: Unexpected findings from the synthesis of benzoxazole derivatives raise new questions on the risks of orthogonal riboswitch design ===
	** http://www.ncbi.nlm.nih.gov/nuccore/NM_003775.3 ''"GeneRIF: findings highlight an important role for S1P(4) and SK1 in ER(-) breast cancer progression."''		** http://www.ncbi.nlm.nih.gov/nuccore/NM_003775.3 ''"GeneRIF: findings highlight an important role for S1P(4) and SK1 in ER(-) breast cancer progression."''
	** ''"Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer."'' http://www.ncbi.nlm.nih.gov/protein/NP_003766.1		** ''"Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer."'' http://www.ncbi.nlm.nih.gov/protein/NP_003766.1
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	** http://omim.org/entry/603751		** http://omim.org/entry/603751
	** http://omim.org/entry/601974		** http://omim.org/entry/601974
-	** google key words: ~~benzodiazepine, oxazole~~, CAMK2B, S1PR4 (Breast cancer)	+	** google key words: benzoxazole, CAMK2B, S1PR4 (Breast cancer)
	** Horn-directed "S1PR4" mutant protein amplification and overexpression (Calmodulin/Rhodopsin-dependent orthogonal riboswitch): http://www.uniprot.org/uniprot/O95977		** Horn-directed "S1PR4" mutant protein amplification and overexpression (Calmodulin/Rhodopsin-dependent orthogonal riboswitch): http://www.uniprot.org/uniprot/O95977
		+
	=== Light (round the horn) directed S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12) ===		=== Light (round the horn) directed S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12) ===
	* Breast cancer/Alzheimer/Leukemia (DS, Parkinson, etc): Rhodopsin (Rho) gene-level interactions with <font color=red>S1PR4-directed</font> protein overexpression lineage in HEK293 important note: <font color="red">''The Rho signalling pathway is activated, for example, by bioactive sphingolipids such as sphingosine-1-phosphate (SPP) or by overexpression of Rho family members in tumorigenesis and metastases [16].'' </font> [Note: see [[User:Etienne_Robillard/Notebook/Rhodopsin\|Rhodopsin]] for info on photophosphorylation of the Rhodopsin kinase, a Ca2-dependent photoreceptor/GPCR]		* Breast cancer/Alzheimer/Leukemia (DS, Parkinson, etc): Rhodopsin (Rho) gene-level interactions with <font color=red>S1PR4-directed</font> protein overexpression lineage in HEK293 important note: <font color="red">''The Rho signalling pathway is activated, for example, by bioactive sphingolipids such as sphingosine-1-phosphate (SPP) or by overexpression of Rho family members in tumorigenesis and metastases [16].'' </font> [Note: see [[User:Etienne_Robillard/Notebook/Rhodopsin\|Rhodopsin]] for info on photophosphorylation of the Rhodopsin kinase, a Ca2-dependent photoreceptor/GPCR]

Etienne Robillard at 00:57, 30 March 2013

Etienne Robillard — Sat, 30 Mar 2013 00:57:56 GMT

←Older revision		Revision as of 00:57, 30 March 2013
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	** google key words: benzodiazepine, oxazole, CAMK2B, S1PR4 (Breast cancer)		** google key words: benzodiazepine, oxazole, CAMK2B, S1PR4 (Breast cancer)
	** Horn-directed "S1PR4" mutant protein amplification and overexpression (Calmodulin/Rhodopsin-dependent orthogonal riboswitch): http://www.uniprot.org/uniprot/O95977		** Horn-directed "S1PR4" mutant protein amplification and overexpression (Calmodulin/Rhodopsin-dependent orthogonal riboswitch): http://www.uniprot.org/uniprot/O95977
-	=== Light (horn) ~~activated~~ S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12) ===	+	=== Light (round the horn) directed S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12) ===
	* Breast cancer/Alzheimer/Leukemia (DS, Parkinson, etc): Rhodopsin (Rho) gene-level interactions with <font color=red>S1PR4-directed</font> protein overexpression lineage in HEK293 important note: <font color="red">''The Rho signalling pathway is activated, for example, by bioactive sphingolipids such as sphingosine-1-phosphate (SPP) or by overexpression of Rho family members in tumorigenesis and metastases [16].'' </font> [Note: see [[User:Etienne_Robillard/Notebook/Rhodopsin\|Rhodopsin]] for info on photophosphorylation of the Rhodopsin kinase, a Ca2-dependent photoreceptor/GPCR]		* Breast cancer/Alzheimer/Leukemia (DS, Parkinson, etc): Rhodopsin (Rho) gene-level interactions with <font color=red>S1PR4-directed</font> protein overexpression lineage in HEK293 important note: <font color="red">''The Rho signalling pathway is activated, for example, by bioactive sphingolipids such as sphingosine-1-phosphate (SPP) or by overexpression of Rho family members in tumorigenesis and metastases [16].'' </font> [Note: see [[User:Etienne_Robillard/Notebook/Rhodopsin\|Rhodopsin]] for info on photophosphorylation of the Rhodopsin kinase, a Ca2-dependent photoreceptor/GPCR]
	* Questions/Comments:		* Questions/Comments:

Etienne Robillard: /* Light (horn) activated S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12) */

Etienne Robillard — Fri, 29 Mar 2013 13:40:06 GMT

Light (horn) activated S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12)

←Older revision		Revision as of 13:40, 29 March 2013
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	# What is the technique (protocol name) name the "biological programmers" may have implemented to induce synthetic neurological circuits or programs? -[[User:Etienne_Robillard\|ER]]		# What is the technique (protocol name) name the "biological programmers" may have implemented to induce synthetic neurological circuits or programs? -[[User:Etienne_Robillard\|ER]]
	# Based on your research, what is a neuronal aptamer primary biological functions and describe the key concepts and techniques in the "hijacking" of human DNA as a information storage transcriptional '''vector''' -[[User:Etienne_Robillard\|ER]]		# Based on your research, what is a neuronal aptamer primary biological functions and describe the key concepts and techniques in the "hijacking" of human DNA as a information storage transcriptional '''vector''' -[[User:Etienne_Robillard\|ER]]
-	~~# Note~~: To obtain the consensus pattern, see Accession number PS00237 [http://prosite.expasy.org/PS00237 here]. ([http://prosite.expasy.org/cgi-bin/aligner?psa=PS00237&format=FASTA FASTA]).	+	* Notes:
-	# ~~View the ligands binding [~~http://www.ebi.ac.uk/pdbe-site/pdbemotif/barChartPattern?pattern1=PS50262 ~~here]~~	+	# To obtain the consensus pattern, see Accession number PS00237 [http://prosite.expasy.org/PS00237 here]. ([http://prosite.expasy.org/cgi-bin/aligner?psa=PS00237&format=FASTA FASTA]).
		+	# Ligands/motifs bindings: http://www.ebi.ac.uk/pdbe-site/pdbemotif/barChartPattern?pattern1=PS50262
		+	# Uniprot dataset (S1PR4 g-receptor): http://www.uniprot.org/uniprot/O95977

	=== References ===		=== References ===

Etienne Robillard: /* Light (horn) activated S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12) */

Etienne Robillard — Fri, 29 Mar 2013 13:17:31 GMT

Light (horn) activated S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12)

←Older revision		Revision as of 13:17, 29 March 2013
Line 52:		Line 52:
	# Based on your research, what is a neuronal aptamer primary biological functions and describe the key concepts and techniques in the "hijacking" of human DNA as a information storage transcriptional '''vector''' -[[User:Etienne_Robillard\|ER]]		# Based on your research, what is a neuronal aptamer primary biological functions and describe the key concepts and techniques in the "hijacking" of human DNA as a information storage transcriptional '''vector''' -[[User:Etienne_Robillard\|ER]]
	# Note: To obtain the consensus pattern, see Accession number PS00237 [http://prosite.expasy.org/PS00237 here]. ([http://prosite.expasy.org/cgi-bin/aligner?psa=PS00237&format=FASTA FASTA]).		# Note: To obtain the consensus pattern, see Accession number PS00237 [http://prosite.expasy.org/PS00237 here]. ([http://prosite.expasy.org/cgi-bin/aligner?psa=PS00237&format=FASTA FASTA]).
		+	# View the ligands binding [http://www.ebi.ac.uk/pdbe-site/pdbemotif/barChartPattern?pattern1=PS50262 here]

	=== References ===		=== References ===

Etienne Robillard: /* Light (horn) activated S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12) */

Etienne Robillard — Fri, 29 Mar 2013 13:04:52 GMT

Light (horn) activated S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12)

←Older revision		Revision as of 13:04, 29 March 2013
Line 51:		Line 51:
	# What is the technique (protocol name) name the "biological programmers" may have implemented to induce synthetic neurological circuits or programs? -[[User:Etienne_Robillard\|ER]]		# What is the technique (protocol name) name the "biological programmers" may have implemented to induce synthetic neurological circuits or programs? -[[User:Etienne_Robillard\|ER]]
	# Based on your research, what is a neuronal aptamer primary biological functions and describe the key concepts and techniques in the "hijacking" of human DNA as a information storage transcriptional '''vector''' -[[User:Etienne_Robillard\|ER]]		# Based on your research, what is a neuronal aptamer primary biological functions and describe the key concepts and techniques in the "hijacking" of human DNA as a information storage transcriptional '''vector''' -[[User:Etienne_Robillard\|ER]]
-	# Note: To obtain the consensus pattern, see Accession number PS00237 [http://prosite.expasy.org/PS00237 here].	+	# Note: To obtain the consensus pattern, see Accession number PS00237 [http://prosite.expasy.org/PS00237 here]. ([http://prosite.expasy.org/cgi-bin/aligner?psa=PS00237&format=FASTA FASTA]).

	=== References ===		=== References ===

Etienne Robillard: /* Light (horn) activated S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12) */

Etienne Robillard — Fri, 29 Mar 2013 13:01:20 GMT

Light (horn) activated S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12)

←Older revision		Revision as of 13:01, 29 March 2013
Line 48:		Line 48:
	* Breast cancer/Alzheimer/Leukemia (DS, Parkinson, etc): Rhodopsin (Rho) gene-level interactions with <font color=red>S1PR4-directed</font> protein overexpression lineage in HEK293 important note: <font color="red">''The Rho signalling pathway is activated, for example, by bioactive sphingolipids such as sphingosine-1-phosphate (SPP) or by overexpression of Rho family members in tumorigenesis and metastases [16].'' </font> [Note: see [[User:Etienne_Robillard/Notebook/Rhodopsin\|Rhodopsin]] for info on photophosphorylation of the Rhodopsin kinase, a Ca2-dependent photoreceptor/GPCR]		* Breast cancer/Alzheimer/Leukemia (DS, Parkinson, etc): Rhodopsin (Rho) gene-level interactions with <font color=red>S1PR4-directed</font> protein overexpression lineage in HEK293 important note: <font color="red">''The Rho signalling pathway is activated, for example, by bioactive sphingolipids such as sphingosine-1-phosphate (SPP) or by overexpression of Rho family members in tumorigenesis and metastases [16].'' </font> [Note: see [[User:Etienne_Robillard/Notebook/Rhodopsin\|Rhodopsin]] for info on photophosphorylation of the Rhodopsin kinase, a Ca2-dependent photoreceptor/GPCR]
	* Questions/Comments:		* Questions/Comments:
-	# 1. Which light-activated (AHL) synthetic transcription promoters the DNA engineers may have chosen to induce Calmodulin-dependent '''psychotic''' behavior and <font color=blue>visual hallucinations</font> as the primary phenotype? -[[User:Etienne_Robillard\|ER]]	+	# Which light-activated (AHL) synthetic transcription promoters the DNA engineers may have chosen to induce Calmodulin-dependent '''psychotic''' behavior and <font color=blue>visual hallucinations</font> as the primary phenotype? -[[User:Etienne_Robillard\|ER]]
-	# 2. What is the technique (protocol name) name the "biological programmers" may have implemented to induce synthetic neurological circuits or programs? -[[User:Etienne_Robillard\|ER]]	+	# What is the technique (protocol name) name the "biological programmers" may have implemented to induce synthetic neurological circuits or programs? -[[User:Etienne_Robillard\|ER]]
-	# 3. Based on your research, what is a neuronal aptamer primary biological functions and describe the key concepts and techniques in the "hijacking" of human DNA as a information storage transcriptional '''vector''' -[[User:Etienne_Robillard\|ER]]	+	# Based on your research, what is a neuronal aptamer primary biological functions and describe the key concepts and techniques in the "hijacking" of human DNA as a information storage transcriptional '''vector''' -[[User:Etienne_Robillard\|ER]]
		+	# Note: To obtain the consensus pattern, see Accession number PS00237 [http://prosite.expasy.org/PS00237 here].

	=== References ===		=== References ===