User:Etienne Robillard/Notebook/30C6HSL/2013/03/18

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(Introduction to CAMK2B protein overexpression)
(Clinical significance of exogenous promoters (30C6HSL) in the morphogenesis of Breast cancer (erbb2), CES, Parkinson, and Alzheimer diseases via site-directed signaling (mutagenesis))
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== Long-term effects of aerosol spraying on E. coli K-12 (MG1655) ==  
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<blockquote>"''I submit that an individual who breaks a law that conscience tells him is unjust, and who willingly accepts the penalty of imprisonment in order to arouse the conscience of  the community over its injustice, is in reality expressing the highest respect for the law.''"
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- Martin Luther King Jr</blockquote>
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== Unofficial Agent E. coli (K12, BL21(DES3), MG1655) synthetic recombination cheat sheet with an emphasis on post-transcriptional calcium binding riboswitches ==
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* Long-term effects of calcium signaling  on neuronal growth ? [omim] Dude wheres my axon? [calcium signaling pathways] [axon guidance] [omim] Long-term effects of calcium signaling  on neuronal growth ? [omim] Dude wheres my axon? [calcium signaling pathways] [axon guidance] [omim] Long-term effects of calcium signaling  on neuronal growth ? [omim] Dude wheres my axon? [calcium signaling pathways] [axon guidance] [omim] Long-term effects of calcium signaling  on neuronal growth ? [omim] Dude wheres my axon? [calcium signaling pathways] [axon guidance] [omim]Long-term effects of calcium signaling  on neuronal growth ? [omim] Dude wheres my axon? [calcium signaling pathways] [axon guidance] [omim]
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__TOC__
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=== Introduction to CAMK2B protein overexpression ===
=== Introduction to CAMK2B protein overexpression ===
* examine the acquisition and mutation of the CAMK2B gene in humans subject to horizontal 30C6HSL based transcription promoters (or "devices" for eugenic doctors).  
* examine the acquisition and mutation of the CAMK2B gene in humans subject to horizontal 30C6HSL based transcription promoters (or "devices" for eugenic doctors).  
-
* Calmodulin dependent inverse/split-pericams and CAMK2B interactions: CAMK2B may depend on exogenous promoters to convey the drug at the right time/place (site-directed mutagenesis). Notice that CAMK2B overexpression is probably an indicator of low neuronal activity (inverse pericams) or osmolitic regulator. (Critical sites: brain (frontal cortex region), amygdala and perhaps the hypothalamus)
+
* Calmodulin dependent inverse/split-pericams and CAMK2B interactions: CAMK2B may depend on exogenous promoters to convey the drug at the right time/place (site-directed mutagenesis). Notice that CAMK2B overexpression is probably an indicator of low neuronal activity (inverse pericams) or osmolitic regulator. (Critical sites: brain (frontal cortex region), amygdala and perhaps the hippocampus, a critical site for memory related brain functions.)
* Never forget atmospheric NH2 as natural source of Nitrogen, hence a ethyl-methyl carbanion may be conjugated to a NH2 molecule in a process known as "benzoin condensation" to produce crystals (N-substituted benzoxazole heterocyclics), thus a form of artificial (asymmetric) [[User:Etienne_Robillard/Notebook/Schiff_base|Schiff base synthesis]] may occur as the result of injection of psychochemical intermediates into the atmosphere.
* Never forget atmospheric NH2 as natural source of Nitrogen, hence a ethyl-methyl carbanion may be conjugated to a NH2 molecule in a process known as "benzoin condensation" to produce crystals (N-substituted benzoxazole heterocyclics), thus a form of artificial (asymmetric) [[User:Etienne_Robillard/Notebook/Schiff_base|Schiff base synthesis]] may occur as the result of injection of psychochemical intermediates into the atmosphere.
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=== A synthetic biology approach to light-activated protein delivery ===
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=== A synthetic biology approach to light-activated protein delivery : A review of recent advancements in synthetically-assisted mind kontrol and monitoring ===
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* 2005 - The search for scFV osmolitic dimer -> ompC dimer protein:  [http://www.uniprot.org/uniprot/P06996.fasta P06996] [[http://openwetware.org/wiki/IGEM:MIT/2005 1]], [[http://www.ncbi.nlm.nih.gov/protein/56783237 2]], [[http://www.ncbi.nlm.nih.gov/nucleotide/49175990 3]]
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* 2006 - The eau de coli experiment : ''Would E. coli, by any other name, smell as sweet?”''' : http://www.medgadget.com/2006/11/would_e_coli_by_1.html [http://openwetware.org/wiki/IGEM:MIT/2006 1]
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** leucine auxotrophic gene insert -> osmY http://www.uniprot.org/uniprot/P0AFH8
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** ''Complex transcriptional control of the sigma s-dependent stationary-phase-induced and osmotically regulated osmY (csi-5) gene suggests <font color="red">novel roles for Lrp, cyclic AMP (cAMP) receptor protein-cAMP complex, and integration host factor in the stationary-phase response of Escherichia coli.''</font> http://www.ncbi.nlm.nih.gov/pubmed/8253679
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*** Leucine-responsive regulatory protein (in E.coli K12): See http://www.uniprot.org/uniprot/P0ACJ0
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*** http://www.ncbi.nlm.nih.gov/nuccore/D11105 - Link to FASTA sequence file is [http://www.ncbi.nlm.nih.gov/nuccore/216585?report=fasta here].
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* 2007 - <font class=TTTTAAA COLOR=Purple STYLE="BACKGROUND-COLOR:#ddd"><b EC=2.7.11.11>"Reversible acyl-homoserine lactone (AHL) <font color=red>binding</font> to purified Vibrio fischeri '''LuxR''' protein.''</b></font>
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** <u><font color=purple>''"Recovering patients often suffer hallucinations, and sometimes become psychotic</font>"''</u> : http://www.openwetware.org/wiki/Image:IGEM2007_Imperial_iGEMGlobe.png
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* 2008 -
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* 2009 -
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* 2010 - '''Unknown phenotype'''.  Homo sapiens (GRCh37.p10), s288c (Baker Yeast), E.coli BL21(DES3), E. coli, E. coli K12 (MG1655), W3110  : ''Berkeley Lab scientists open electrical link to living cells'' : http://www.eurekalert.org/pub_releases/2010-10/dbnl-bls102010.php
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* 2013 - <font color=magenta>"''5 Disorders Share Genetic Risk Factors, Study Finds''"</font>: http://www.nytimes.com/2013/03/01/health/study-finds-genetic-risk-factors-shared-by-5-psychiatric-disorders.html
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=== Calmodulin kinase type II Beta subunit protein acquisition and expression with 30C6HSL based exogenous promoters (E. coli K12) ===
 +
* GO:1901896 positive regulation of calcium-transporting ATPase activity: http://www.ebi.ac.uk/QuickGO/GTerm?id=GO:1901896
 +
* GO:0005513 detection of calcium ion: http://www.ebi.ac.uk/QuickGO/GTerm?id=GO:0005513
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* GO:0006970 (osmY) : http://www.ebi.ac.uk/QuickGO/GTerm?id=GO:0006970
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** Co-occuring terms/annotations for "osmY" are found [http://www.ebi.ac.uk/QuickGO/GTerm?id=GO:0006970#term=stats here].
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* Significance of CAMK2B gene acquisition and amplification with exogenous 30C6HSL promoters : ''Within the rigid <font color="red">“β -can” structure (6) of GFP variants</font>, Baird et al. (7) found a site that would tolerate circular permutations where two portions of the polypeptide are flipped around the central site. With obvious clefts in the β-can, the chromophore of the circularly permuted GFPs (cpGFP) seemed to be <font color="red">more accessible to protons outside of the proteins</font>. The use of cpGFP might be interesting for converting the interaction signal between two protein domains into a change in the electrostatic potential of the chromophore; <font color="red">in other words, to transduce the information of the interaction into a fluorescent signal.</font>'' [[http://www.pnas.org/content/98/6/3197.full?sid=abc32731-4df0-45c4-bf46-7863678fb87c 1]]
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** '''Important Note''': THIS IS NOT THE SAME NAGAI et al... The other one has DOI: 10.1038/73767. -[[User:Etienne_Robillard|ER]]
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* Browse the annotations for the Yeast (CMK1) ligand [http://www.uniprot.org/uniprot/P27466 here]. (EC 2.7.11.17)
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=== CAMK2B based protein dimering and expression with 30C6HSL based promoters (LuxR) ===
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=== Clinical significance of exogenous promoters (30C6HSL) in the morphogenesis of Breast cancer (erbb2), CES, Parkinson, and Alzheimer diseases via site-directed signaling (mutagenesis) ===
 +
* Brain disorders : patient become mentally ill/unstable (<font color=red>psychotic</font>) and unable to express clear/rational thoughs
 +
* Visual hallucinations (see Brain disorders above)
 +
* intellect skills degradation (ie the difficulty to learn, remember or acquire new learnings..)
 +
* agressivity (esp. in male subjects)
 +
* selective mutism (esp. in female subjects)
 +
* In male subjects - alcoholism like symptoms (ref: [http://brenda-enzymes.org/php/result_flat.php4?ecno=1.1.1.1&Suchword=&organism%5B%5D=Escherichia+coli&show_tm=0 aldehyde dehydrogenase - EC 1.1.1.1])
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=== Motivations and purposes of psychochemical spraying in the morphogenesis of breast cancer, CES, Parkinson, and Alzheimer diseases via site-directed signaling (mutagenesis) ===
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== Selected Paper list ==
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<biblio>
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#LuxR_operon1 pmid=14729687
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#Ugi_route_7c Weber, W. P., Gokel, G. W. and Ugi, I. K. (1972), Phase Transfer Catalysis in the Hofmann Carbylamine Reaction. Angew. Chem. Int. Ed. Engl., 11: 530–531. [[doi:10.1002/anie.197205301|Paper]]
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// Ugi route to Wilmslow... (Take route 7c, then pick MNR-8-5 highway to avoid Traffic!!!) :)
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#Isocyanide1 "Recent Developments in Isocyanide Based Multicomponent Reactions in Applied Chemistry" http://pubs.acs.org/doi/abs/10.1021/cr0505728
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#MDM2_P53_Interactions pmid=8875929
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</biblio>

Revision as of 08:17, 6 May 2013

"I submit that an individual who breaks a law that conscience tells him is unjust, and who willingly accepts the penalty of imprisonment in order to arouse the conscience of the community over its injustice, is in reality expressing the highest respect for the law." - Martin Luther King Jr

Unofficial Agent E. coli (K12, BL21(DES3), MG1655) synthetic recombination cheat sheet with an emphasis on post-transcriptional calcium binding riboswitches

  • Long-term effects of calcium signaling on neuronal growth ? [omim] Dude wheres my axon? [calcium signaling pathways] [axon guidance] [omim] Long-term effects of calcium signaling on neuronal growth ? [omim] Dude wheres my axon? [calcium signaling pathways] [axon guidance] [omim] Long-term effects of calcium signaling on neuronal growth ? [omim] Dude wheres my axon? [calcium signaling pathways] [axon guidance] [omim] Long-term effects of calcium signaling on neuronal growth ? [omim] Dude wheres my axon? [calcium signaling pathways] [axon guidance] [omim]Long-term effects of calcium signaling on neuronal growth ? [omim] Dude wheres my axon? [calcium signaling pathways] [axon guidance] [omim]


Contents


Introduction to CAMK2B protein overexpression

  • examine the acquisition and mutation of the CAMK2B gene in humans subject to horizontal 30C6HSL based transcription promoters (or "devices" for eugenic doctors).
  • Calmodulin dependent inverse/split-pericams and CAMK2B interactions: CAMK2B may depend on exogenous promoters to convey the drug at the right time/place (site-directed mutagenesis). Notice that CAMK2B overexpression is probably an indicator of low neuronal activity (inverse pericams) or osmolitic regulator. (Critical sites: brain (frontal cortex region), amygdala and perhaps the hippocampus, a critical site for memory related brain functions.)
  • Never forget atmospheric NH2 as natural source of Nitrogen, hence a ethyl-methyl carbanion may be conjugated to a NH2 molecule in a process known as "benzoin condensation" to produce crystals (N-substituted benzoxazole heterocyclics), thus a form of artificial (asymmetric) Schiff base synthesis may occur as the result of injection of psychochemical intermediates into the atmosphere.

A synthetic biology approach to light-activated protein delivery : A review of recent advancements in synthetically-assisted mind kontrol and monitoring

Calmodulin kinase type II Beta subunit protein acquisition and expression with 30C6HSL based exogenous promoters (E. coli K12)

  • GO:1901896 positive regulation of calcium-transporting ATPase activity: http://www.ebi.ac.uk/QuickGO/GTerm?id=GO:1901896
  • GO:0005513 detection of calcium ion: http://www.ebi.ac.uk/QuickGO/GTerm?id=GO:0005513
  • GO:0006970 (osmY) : http://www.ebi.ac.uk/QuickGO/GTerm?id=GO:0006970
    • Co-occuring terms/annotations for "osmY" are found here.
  • Significance of CAMK2B gene acquisition and amplification with exogenous 30C6HSL promoters : Within the rigid “β -can” structure (6) of GFP variants, Baird et al. (7) found a site that would tolerate circular permutations where two portions of the polypeptide are flipped around the central site. With obvious clefts in the β-can, the chromophore of the circularly permuted GFPs (cpGFP) seemed to be more accessible to protons outside of the proteins. The use of cpGFP might be interesting for converting the interaction signal between two protein domains into a change in the electrostatic potential of the chromophore; in other words, to transduce the information of the interaction into a fluorescent signal. [1]
    • Important Note: THIS IS NOT THE SAME NAGAI et al... The other one has DOI: 10.1038/73767. -ER
  • Browse the annotations for the Yeast (CMK1) ligand here. (EC 2.7.11.17)

Clinical significance of exogenous promoters (30C6HSL) in the morphogenesis of Breast cancer (erbb2), CES, Parkinson, and Alzheimer diseases via site-directed signaling (mutagenesis)

  • Brain disorders : patient become mentally ill/unstable (psychotic) and unable to express clear/rational thoughs
  • Visual hallucinations (see Brain disorders above)
  • intellect skills degradation (ie the difficulty to learn, remember or acquire new learnings..)
  • agressivity (esp. in male subjects)
  • selective mutism (esp. in female subjects)
  • In male subjects - alcoholism like symptoms (ref: aldehyde dehydrogenase - EC 1.1.1.1)

Selected Paper list

  1. Urbanowski ML, Lostroh CP, and Greenberg EP. . pmid:14729687. PubMed HubMed [LuxR_operon1]
  2. Weber, W. P., Gokel, G. W. and Ugi, I. K. (1972), Phase Transfer Catalysis in the Hofmann Carbylamine Reaction. Angew. Chem. Int. Ed. Engl., 11: 530–531. Paper [Ugi_route_7c]
    Ugi route to Wilmslow... (Take route 7c, then pick MNR-8-5 highway to avoid Traffic!!!)  :)

  3. "Recent Developments in Isocyanide Based Multicomponent Reactions in Applied Chemistry" http://pubs.acs.org/doi/abs/10.1021/cr0505728 [Isocyanide1]
  4. Kussie PH, Gorina S, Marechal V, Elenbaas B, Moreau J, Levine AJ, and Pavletich NP. . pmid:8875929. PubMed HubMed [MDM2_P53_Interactions]
All Medline abstracts: PubMed HubMed
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