User:Etienne Robillard/Notebook/Agent Ecoli
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| - | + | * [http://openwetware.org/wiki/Haynes:Gibson_Assembly Gibson Assembly] | |
| - | + | ** "Bill Collins contributed the development of a series of web-based tools for DNA assembly, including Gibthon - a software tool for automated design of the oligonucleotides required for Gibson Assembly." [[http://www.synbio.org.uk/dna-assembly/guidetogibsonassembly.html 1]] [2009-2010, see also the Team:Cambridge website for yet more connections to V. fischeri, a marine organism which expresses bioluminiscence in dark environments.] | |
| - | + | ** Hopefully we sought not to encounter V. fischeri genes into elephants except perhaps into Cambridge, USA: [[http://2010.igem.org/Team:Cambridge/Bioluminescence/G28 2]] | |
=== Forensic evidences of nitrogenous derived gene delivery on domestic civilian populations is a provocative and deliberate act of war on civilian liberties === | === Forensic evidences of nitrogenous derived gene delivery on domestic civilian populations is a provocative and deliberate act of war on civilian liberties === | ||
* Case-study of M13K07 derived genome engineering using site-directed mutagenesis | * Case-study of M13K07 derived genome engineering using site-directed mutagenesis | ||
Revision as of 21:37, 14 November 2012
Agent Ecoli
Synopsis
Long-term bioaerosols spraying on unwary citizens may act as genetic trojan horse for unethical horizontal gene transfer and recombineering on domestic populations in Quebec.
Introduction
[TODO]
Chapters
Introduction to Agent E. coli and chemical warfare
- Definition of Agent E.coli
- Basic description
- Geopolitical background
- Why is the term "chemtrails" inappropriate to describe domestic chemical warfare ?
It is my belief the "chemtrails" term is inappropriate to coherently define the existence of a long-term spraying operation of biologically active aerosols over my city. I believe the "chemtrails" terminology is in addition part of a propaganda scheme to deceit further independent scientists to examine the evidences towards the inclusion of mobile genetic elements being transfered into the human genome.
Agent E. coli mecanics: Fundamental elements of domestic chemical warfare
- Synthetic biology and reverse/forward DNA transcription
- luxR: Introduction to the Phosphohistidine transferase genetic switch
DNA evidences of synthetic nucleosides analogues are in the products
- dual input DNA promoters, heterocyclics, organocatalysts, and phosphoramidates-based nucleosides
- Plasmid addiction systems
- Tools (softwares): Ape (A Plasmid Editor), gibthon (a django-based web application)
- cDNA synthesis (RNAP)
Gibson assembly
- Gibson Assembly
- "Bill Collins contributed the development of a series of web-based tools for DNA assembly, including Gibthon - a software tool for automated design of the oligonucleotides required for Gibson Assembly." [1] [2009-2010, see also the Team:Cambridge website for yet more connections to V. fischeri, a marine organism which expresses bioluminiscence in dark environments.]
- Hopefully we sought not to encounter V. fischeri genes into elephants except perhaps into Cambridge, USA: [2]
Forensic evidences of nitrogenous derived gene delivery on domestic civilian populations is a provocative and deliberate act of war on civilian liberties
- Case-study of M13K07 derived genome engineering using site-directed mutagenesis
