Revision as of 11:29, 27 November 2012

Agent Ecoli: The whys and wherefores of Angel Dust is in the product.

Untested Agent Ecoli 1.0 recipe /protocol:

pick at least a combination of 1 (one) nerve agent (ie Benzoyl);
autoclave custom plasmid(s) with ape... ;
click on "I UNDERSTAND AND ACKNOWLEDGE THE RISKS OF CLONING FLUORESCENT ELEPHANTS, JUST GIMME SOME HARDCORE PLASMIDS TO MAKE MY CUSTOM OLIGOS!";
add water (wash in bath with sodium hydrochloride solution..);
To make a Lysis buffer reagent, add 1 (one) piperidine-hydrochloride derived reagent (ie Tris-HCl);
To add a good source of aceton to your DNA mix use 1 (one) 2-cyanoethyl derived reagent (acetonitrile);
For doing oligos version include 1 (one) organophosphate-based organic phosphatase compound (PHP, P-N, PC) as protection group...
Moreover the DNA methylation process is believed to be regulated by endogenous enzymatic reactions implicated in phosphorus and N degradation.

Keywords: DNA methylation, cDNA, deoxyoligonucleotides, ribosomes, phosphate PHP2-like, Agent E.coli, Benzoyl, PHP

[TODO]

Synthetic biology and reverse/forward DNA transcription "circuits"
- luxR: Introduction to the Phosphohistidine transferase genetic switch
- Agent Ecoli:ToggleSwitchComponent (TL phage lambda)

dual input DNA promoters, heterocyclics, organocatalysts, and phosphoramidates-based nucleosides
Plasmid addiction systems
- Tools (softwares): Ape (A Plasmid Editor), gibthon (a django-based web application)
- cDNA synthesis (RNAP)

Quote from the synbio.org.uk website: "Bill Collins contributed the development of a series of web-based tools for DNA assembly, including Gibthon - a software tool for automated design of the oligonucleotides required for Gibson Assembly." [1]
- [2009-2010. see also the Team:Cambridge website for yet more connections to V. fischeri, a marine organism which expresses bioluminescence in dark environments.]
- Hopefully we sought not to encounter V. fischeri genes into elephants (or fast-running mouses!) except perhaps into Cambridge, USA: [2]
See also Gibson Assembly, pSB3K3, BBa_J69512
In summary, the Gibson assembly protocol provides an automated environment for template DNA synthesis (oligonucleotides) using a web-based interface written in Django for horizontal gene transfers into multi-plasmid cloning vectors such as the pSB3K3 plasmid.

@@ Line 5: / Line 5: @@
 == Overview ==
-Long-term bioaerosols spraying may promote a genetic trojan horse for unethical gene transfer and
+__TOC__
-recombineering on civilian populations in Quebec using chemoselective nerve agents, PCP-derived
-reagents, and organophosphate-based compounds.
+* Long-term bioaerosols spraying (ie TL3')
+* XXX: may carry a '''epigenetic trojan horse''' ?
+* TL = toggle bistable switch / circuit
+=== summary ===
+Untested Agent Ecoli 1.0 recipe /protocol:
+* pick at least a combination of 1 (one) nerve agent (ie Benzoyl);
+* autoclave custom plasmid(s) with ape... ;
+* click on '''"I UNDERSTAND AND ACKNOWLEDGE THE RISKS OF CLONING FLUORESCENT ELEPHANTS, JUST GIMME SOME HARDCORE PLASMIDS TO MAKE MY CUSTOM OLIGOS!";
+* add water (wash in bath with sodium hydrochloride solution..);
+* To make a Lysis buffer reagent, add 1 (one) piperidine-hydrochloride derived reagent (ie Tris-HCl);
+* To add a good source of aceton to your DNA mix use 1 (one) 2-cyanoethyl derived reagent (acetonitrile);
+* For doing oligos version include 1 (one) organophosphate-based organic phosphatase compound (PHP, P-N, PC) as protection group...
+*  Moreover the DNA methylation process is believed to be regulated by endogenous enzymatic reactions implicated in phosphorus and N degradation.
+Keywords: DNA methylation, cDNA, deoxyoligonucleotides, ribosomes, phosphate PHP2-like, Agent E.coli, Benzoyl, PHP
 == Introduction ==