User:Etienne Robillard/Notebook/Agent Ecoli

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(Introduction to Agent E. coli and chemical warfare)
(Agent E. coli mecanics: Fundamental elements of domestic chemical warfare)
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= Agent Ecoli =  
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= Agent Ecoli: The whys and wherefores of Angel Dust is in the product. =  
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'''Notice: This page is outdated - For updates about Agent Ecoli and documentation about 1,2,3,4-tetrahydroisoquinolines derivatives, see [[User:Etienne_Robillard/Notebook/Arylcyclohexylamines|Arylcyclohexylamines]].'''
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== Overview ==
== Overview ==
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Long-term bioaerosols spraying on unwary citizens may act as genetic trojan horse for unethical horizontal gene transfer and
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recombineering on domestic populations in Quebec.
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* Long-term bioaerosols spraying (ie TL3')
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* XXX: may carry a '''epigenetic trojan horse''' ?
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* TL = toggle bistable switch / circuit
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=== summary ===
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Keywords: DNA methylation, cDNA, deoxyoligonucleotides, ribosomes, phosphatase (PAP2-like), Phosphohistidine regulation, Agent Dust, Agent Wet, PCP, Benzoyl, PHP, PC, lysogens, lytic cycle, trojan, horse, epygenetics, elephants, E. coli, K-12, MG1655, Agent Scooby Doo, aspartic acid (NMDA receptor)
== Introduction ==
== Introduction ==
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* [http://www.openwetware.org/wiki/User:Etienne_Robillard/Notebook/Agent_Ecoli:Introduction A beginner introduction to Agent E. coli (Start here)]
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[TODO]
[TODO]
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== Chapters ==
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== Concepts ==
=== Introduction to Agent E. coli ===
=== Introduction to Agent E. coli ===
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=== Agent E. coli mecanics: Fundamental elements of domestic chemical warfare ===
=== Agent E. coli mecanics: Fundamental elements of domestic chemical warfare ===
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* Synthetic biology and reverse/forward DNA transcription
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* Primer 1 : '''Mutant protein delivery and reverse/forward DNA transcription "circuits", light-activated exogenous promoters (luxR)''':
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** luxR: Introduction to the Phosphohistidine transferase genetic switch
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** N-methyl substituted transferase synthetic reagents (specialized O-ribosomes): [[User:Etienne_Robillard/Notebook/30C6HSL|30C6HSL]]
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** Schiff bases, LuxR and RNA transducing Mitogen Activated Protein Kinase (MAPK) specialized proteins, see [[User:Etienne_Robillard/Notebook/Schiff_base|here]] and [http://www.chem.ucalgary.ca/courses/351/Carey5th/Ch25/ch25-3-2.html here].
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* Primer 2 : '''Synthetic transcription/translation functions of Agent E.coli, a light-activated orthogonal riboswitch to induce mutant protein acquisition''' and repression of the envZ gene through Calmodulin-mediated ion (osmolitic) dimers:
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** PHP1T gene (Human phosphotransferase): http://www.uniprot.org/uniprot/Q9NRX4
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** Keywords: Heterocyclic oxazole (carbenes), 30C6HSL, RNA, calmodulin, protein, kinase, cAMP, riboswitch, Imine (Schiff base)
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** Categories: Pharmaceutical intermediates, Exogenous bacterial (type II) promoters, Acyl-Homoserine Lactone, Quorum Sensing, Protein expression
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** By FW and GO accession code: FW:420918
=== DNA evidences of synthetic nucleosides analogues are in the products ===
=== DNA evidences of synthetic nucleosides analogues are in the products ===

Revision as of 10:51, 22 March 2013

Agent Ecoli: The whys and wherefores of Angel Dust is in the product.

Notice: This page is outdated - For updates about Agent Ecoli and documentation about 1,2,3,4-tetrahydroisoquinolines derivatives, see Arylcyclohexylamines.

Contents


Overview

  • Long-term bioaerosols spraying (ie TL3')
  • XXX: may carry a epigenetic trojan horse ?
  • TL = toggle bistable switch / circuit

summary

Keywords: DNA methylation, cDNA, deoxyoligonucleotides, ribosomes, phosphatase (PAP2-like), Phosphohistidine regulation, Agent Dust, Agent Wet, PCP, Benzoyl, PHP, PC, lysogens, lytic cycle, trojan, horse, epygenetics, elephants, E. coli, K-12, MG1655, Agent Scooby Doo, aspartic acid (NMDA receptor)

Introduction


[TODO]

Concepts

Introduction to Agent E. coli

  • Definition of Agent E.coli
    • Basic description
    • Geopolitical background
    • Why is the term "chemtrails" inappropriate to describe domestic chemical warfare ?
      • It is my belief the "chemtrails" term is inappropriate to coherently define the existence of a long-term spraying operation of biologically active aerosols over my city. I believe the "chemtrails" terminology is in addition part of a propaganda scheme to deceit further independent scientists to examine the evidences towards the inclusion of mobile genetic elements being transfered into the human genome.
    • Classification/Taxonomy
      • Agent E.coli 1.0:
        • Class: Nitrogenous-derived nerve precursor agent. Exogenous compound (Military grade). Organophosphate based pesticide (phosphoramidate). Oil-in-water (O/W) nanoemulsion (Lysis Buffer reagents includes Triton X-100, Tween 80, Tris-HCl). Nitrogenous derived heterocyclic carbene (NHC).

Agent E. coli mecanics: Fundamental elements of domestic chemical warfare

  • Primer 1 : Mutant protein delivery and reverse/forward DNA transcription "circuits", light-activated exogenous promoters (luxR):
    • N-methyl substituted transferase synthetic reagents (specialized O-ribosomes): 30C6HSL
    • Schiff bases, LuxR and RNA transducing Mitogen Activated Protein Kinase (MAPK) specialized proteins, see here and here.
  • Primer 2 : Synthetic transcription/translation functions of Agent E.coli, a light-activated orthogonal riboswitch to induce mutant protein acquisition and repression of the envZ gene through Calmodulin-mediated ion (osmolitic) dimers:
    • PHP1T gene (Human phosphotransferase): http://www.uniprot.org/uniprot/Q9NRX4
    • Keywords: Heterocyclic oxazole (carbenes), 30C6HSL, RNA, calmodulin, protein, kinase, cAMP, riboswitch, Imine (Schiff base)
    • Categories: Pharmaceutical intermediates, Exogenous bacterial (type II) promoters, Acyl-Homoserine Lactone, Quorum Sensing, Protein expression
    • By FW and GO accession code: FW:420918

DNA evidences of synthetic nucleosides analogues are in the products

  • dual input DNA promoters, heterocyclics, organocatalysts, and phosphoramidates-based nucleosides
  • Plasmid addiction systems
    • Tools (softwares): Ape (A Plasmid Editor), gibthon (a django-based web application)
    • cDNA synthesis (RNAP)

Gibson assembly method

  • Quote from the synbio.org.uk website: "Bill Collins contributed the development of a series of web-based tools for DNA assembly, including Gibthon - a software tool for automated design of the oligonucleotides required for Gibson Assembly." [1]
    • [2009-2010. see also the Team:Cambridge website for yet more connections to V. fischeri, a marine organism which expresses bioluminescence in dark environments.]
    • Hopefully we sought not to encounter V. fischeri genes into elephants (or fast-running mouses!) except perhaps into Cambridge, USA: [2]
  • See also Gibson Assembly, pSB3K3, BBa_J69512
  • In summary, the Gibson assembly protocol provides an automated environment for template DNA synthesis (oligonucleotides) using a web-based interface written in Django for horizontal gene transfers into multi-plasmid cloning vectors such as the pSB3K3 plasmid.

Forensic evidences of nitrogenous derived gene delivery on domestic civilian populations is a provocative and deliberate act of war on civilian liberties

  • Case-study of M13K07 derived genome engineering using site-directed mutagenesis
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