User:Etienne Robillard/Notebook/Agent Ecoli

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(Agent Ecoli: The whys and wherefores of Angel Dust is in the product.)
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= Agent Ecoli: The whys and wherefores of Angel Dust is in the product. =
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__TOC__
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== Overview ==
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__TOC__
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* Long-term bioaerosols spraying (ie TL3')
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* XXX: may carry a '''epigenetic trojan horse''' ?
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* TL = toggle bistable switch / circuit
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=== summary ===
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Keywords: DNA methylation, cDNA, deoxyoligonucleotides, ribosomes, phosphatase (PAP2-like), Phosphohistidine regulation, Agent Dust, Agent Wet, PCP, Benzoyl, PHP, PC, lysogens, lytic cycle, trojan, horse, epygenetics, elephants, E. coli, K-12, MG1655, Agent Scooby Doo, aspartic acid (NMDA receptor)
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== Introduction ==
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* [http://www.openwetware.org/wiki/User:Etienne_Robillard/Notebook/Agent_Ecoli:Introduction A beginner introduction to Agent E. coli (Start here)]
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[TODO]
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== Concepts ==
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=== Introduction to Agent E. coli ===
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* Definition of Agent E.coli
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** Basic description
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** Geopolitical background
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** Why is the term "chemtrails" inappropriate to describe domestic chemical warfare ?
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*** It is my belief the "chemtrails" term is inappropriate to coherently define the existence of a long-term spraying operation of biologically active aerosols over my city. I believe the "chemtrails" terminology is in addition part of a propaganda scheme to deceit further independent scientists to examine the evidences towards the inclusion of mobile genetic elements being transfered into the human genome.
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** Classification/Taxonomy
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*** '''Agent E.coli 1.0''':
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**** Class:  Nitrogenous-derived [http://en.wikipedia.org/wiki/Nerve_agent nerve precursor agent]. Exogenous compound (Military grade). Organophosphate based pesticide (phosphoramidate). Oil-in-water (O/W) nanoemulsion (Lysis Buffer reagents includes Triton X-100, Tween 80, Tris-HCl). Nitrogenous derived heterocyclic carbene (NHC).
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=== Agent E. coli mecanics: Fundamental elements of domestic chemical warfare ===
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* Synthetic biology and reverse/forward DNA transcription "circuits"
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** luxR: Introduction to the Phosphohistidine transferase genetic switch
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** [http://openwetware.org/index.php?title=User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent Agent Ecoli:ToggleSwitchComponent] (TL phage lambda)
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=== DNA evidences of synthetic nucleosides analogues are in the products ===
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* dual input DNA promoters, heterocyclics, organocatalysts, and phosphoramidates-based nucleosides
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* Plasmid addiction systems
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** Tools (softwares): Ape (A Plasmid Editor), gibthon (a django-based web application)
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** cDNA synthesis (RNAP)
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==== Gibson assembly method ====
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* Quote from the synbio.org.uk website: "Bill Collins contributed the development of a series of web-based tools for DNA assembly, including Gibthon - a software tool for automated design of the oligonucleotides required for Gibson Assembly." [[http://www.synbio.org.uk/dna-assembly/guidetogibsonassembly.html 1]]
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** [2009-2010. see also the Team:Cambridge website for yet more connections to V. fischeri, a marine organism which expresses bioluminescence in dark environments.]
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** Hopefully we sought not to encounter V. fischeri genes into elephants (or fast-running mouses!) except perhaps into Cambridge, USA: [[http://2010.igem.org/Team:Cambridge/Bioluminescence/G28 2]]
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* See also [http://openwetware.org/wiki/Haynes:Gibson_Assembly Gibson Assembly], pSB3K3, BBa_J69512
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* In summary, the Gibson assembly protocol provides an automated environment for template DNA synthesis (oligonucleotides) using a web-based interface written in Django for horizontal gene transfers into multi-plasmid cloning vectors such as the '''pSB3K3''' plasmid.
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=== Forensic evidences of nitrogenous derived gene delivery on domestic civilian populations is a provocative and deliberate act of war on civilian liberties  ===
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* Case-study of M13K07 derived genome engineering using site-directed mutagenesis
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