User:Etienne Robillard/Notebook/Agent Ecoli - Revision history

Etienne Robillard: /* Agent E. coli mecanics: Fundamental elements of domestic chemical warfare */

Etienne Robillard — Fri, 22 Mar 2013 15:51:52 GMT

Agent E. coli mecanics: Fundamental elements of domestic chemical warfare

←Older revision		Revision as of 15:51, 22 March 2013
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	** N-methyl substituted transferase synthetic reagents (specialized O-ribosomes): [[User:Etienne_Robillard/Notebook/30C6HSL\|30C6HSL]]		** N-methyl substituted transferase synthetic reagents (specialized O-ribosomes): [[User:Etienne_Robillard/Notebook/30C6HSL\|30C6HSL]]
	** Schiff bases, LuxR and RNA transducing Mitogen Activated Protein Kinase (MAPK) specialized proteins, see [[User:Etienne_Robillard/Notebook/Schiff_base\|here]] and [http://www.chem.ucalgary.ca/courses/351/Carey5th/Ch25/ch25-3-2.html here].		** Schiff bases, LuxR and RNA transducing Mitogen Activated Protein Kinase (MAPK) specialized proteins, see [[User:Etienne_Robillard/Notebook/Schiff_base\|here]] and [http://www.chem.ucalgary.ca/courses/351/Carey5th/Ch25/ch25-3-2.html here].
-	* Primer 2 : '''Synthetic transcription/translation functions of Agent E.coli, a light-activated orthogonal riboswitch to induce mutant protein acquisition'''	+	* Primer 2 : '''Synthetic transcription/translation functions of Agent E.coli, a light-activated orthogonal riboswitch to induce mutant protein acquisition''' and repression of the envZ gene through Calmodulin-mediated ion (osmolitic) dimers:
-	and repression of the envZ gene through Calmodulin-mediated ion (osmolitic) dimers:	+
-	** [[User:Etienne_Robillard/Notebook/Agent_Ecoli\|AgentEcoli:ToggleSwitchComponent]]	+
	** PHP1T gene (Human phosphotransferase): http://www.uniprot.org/uniprot/Q9NRX4		** PHP1T gene (Human phosphotransferase): http://www.uniprot.org/uniprot/Q9NRX4
	** Keywords: Heterocyclic oxazole (carbenes), 30C6HSL, RNA, calmodulin, protein, kinase, cAMP, riboswitch, Imine (Schiff base)		** Keywords: Heterocyclic oxazole (carbenes), 30C6HSL, RNA, calmodulin, protein, kinase, cAMP, riboswitch, Imine (Schiff base)

Etienne Robillard: /* Agent E. coli mecanics: Fundamental elements of domestic chemical warfare */

Etienne Robillard — Fri, 22 Mar 2013 15:47:01 GMT

Agent E. coli mecanics: Fundamental elements of domestic chemical warfare

←Older revision		Revision as of 15:47, 22 March 2013
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	=== Agent E. coli mecanics: Fundamental elements of domestic chemical warfare ===		=== Agent E. coli mecanics: Fundamental elements of domestic chemical warfare ===

-	* ~~Synthetic biology~~ and reverse/forward DNA transcription "circuits"	+	* Primer 1 : '''Mutant protein delivery and reverse/forward DNA transcription "circuits", light-activated exogenous promoters (luxR)''':
-	** ~~luxR: Introduction to the Phosphohistidine~~ N-~~methyltransferase~~ O-~~riboswitch~~: [[User:Etienne_Robillard/Notebook/30C6HSL\|~~a type of~~ RNA transducing Mitogen Activated Protein Kinase (MAPK) ~~using an imine as the drug carrier~~]]~~. (a Serine-Threonine specific kinase)~~ [http://www.~~uniprot~~.~~org~~/~~uniprot~~/~~Q9NRX4 1~~]	+	** N-methyl substituted transferase synthetic reagents (specialized O-ribosomes): [[User:Etienne_Robillard/Notebook/30C6HSL\|30C6HSL]]
-	** [http:/~~/openwetware~~.~~org/index.php?title=~~User:Etienne_Robillard/Notebook/Agent_Ecoli~~:ToggleSwitchComponent Agent Ecoli~~:ToggleSwitchComponent] (~~TL phage lambda~~)	+	** Schiff bases, LuxR and RNA transducing Mitogen Activated Protein Kinase (MAPK) specialized proteins, see [[User:Etienne_Robillard/Notebook/Schiff_base\|here]] and [http://www.chem.ucalgary.ca/courses/351/Carey5th/Ch25/ch25-3-2.html here].
		+	* Primer 2 : '''Synthetic transcription/translation functions of Agent E.coli, a light-activated orthogonal riboswitch to induce mutant protein acquisition'''
		+	and repression of the envZ gene through Calmodulin-mediated ion (osmolitic) dimers:
		+	** [[User:Etienne_Robillard/Notebook/Agent_Ecoli\|AgentEcoli:ToggleSwitchComponent]]
		+	** PHP1T gene (Human phosphotransferase): http://www.uniprot.org/uniprot/Q9NRX4
		+	** Keywords: Heterocyclic oxazole (carbenes), 30C6HSL, RNA, calmodulin, protein, kinase, cAMP, riboswitch, Imine (Schiff base)
		+	** Categories: Pharmaceutical intermediates, Exogenous bacterial (type II) promoters, Acyl-Homoserine Lactone, Quorum Sensing, Protein expression
		+	** By FW and GO accession code: FW:420918

	=== DNA evidences of synthetic nucleosides analogues are in the products ===		=== DNA evidences of synthetic nucleosides analogues are in the products ===

Etienne Robillard: /* Agent E. coli mecanics: Fundamental elements of domestic chemical warfare */

Etienne Robillard — Fri, 22 Mar 2013 13:42:10 GMT

Agent E. coli mecanics: Fundamental elements of domestic chemical warfare

←Older revision		Revision as of 13:42, 22 March 2013
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	* Synthetic biology and reverse/forward DNA transcription "circuits"		* Synthetic biology and reverse/forward DNA transcription "circuits"
-	** luxR: Introduction to the Phosphohistidine N-methyltransferase riboswitch: [[User:Etienne_Robillard/Notebook/30C6HSL\|a type of RNA transducing Mitogen Activated Protein Kinase (MAPK) using an imine as the drug carrier]]. (a Serine-Threonine specific kinase) [http://www.uniprot.org/uniprot/Q9NRX4 1]	+	** luxR: Introduction to the Phosphohistidine N-methyltransferase O-riboswitch: [[User:Etienne_Robillard/Notebook/30C6HSL\|a type of RNA transducing Mitogen Activated Protein Kinase (MAPK) using an imine as the drug carrier]]. (a Serine-Threonine specific kinase) [http://www.uniprot.org/uniprot/Q9NRX4 1]
	** [http://openwetware.org/index.php?title=User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent Agent Ecoli:ToggleSwitchComponent] (TL phage lambda)		** [http://openwetware.org/index.php?title=User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent Agent Ecoli:ToggleSwitchComponent] (TL phage lambda)

Etienne Robillard: /* Agent E. coli mecanics: Fundamental elements of domestic chemical warfare */

Etienne Robillard — Wed, 13 Mar 2013 22:34:01 GMT

Agent E. coli mecanics: Fundamental elements of domestic chemical warfare

←Older revision		Revision as of 22:34, 13 March 2013
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	* Synthetic biology and reverse/forward DNA transcription "circuits"		* Synthetic biology and reverse/forward DNA transcription "circuits"
-	** luxR: Introduction to the Phosphohistidine N-methyltransferase riboswitch: [[User:Etienne_Robillard/Notebook/30C6HSL\|a type of RNA transducing Mitogen Activated Protein Kinase (MAPK) using an imine as the drug carrier]]. (a ~~serine~~-~~threonine~~ specific kinase) [http://www.uniprot.org/uniprot/Q9NRX4 1]	+	** luxR: Introduction to the Phosphohistidine N-methyltransferase riboswitch: [[User:Etienne_Robillard/Notebook/30C6HSL\|a type of RNA transducing Mitogen Activated Protein Kinase (MAPK) using an imine as the drug carrier]]. (a Serine-Threonine specific kinase) [http://www.uniprot.org/uniprot/Q9NRX4 1]
	** [http://openwetware.org/index.php?title=User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent Agent Ecoli:ToggleSwitchComponent] (TL phage lambda)		** [http://openwetware.org/index.php?title=User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent Agent Ecoli:ToggleSwitchComponent] (TL phage lambda)

Etienne Robillard: /* Agent E. coli mecanics: Fundamental elements of domestic chemical warfare */

Etienne Robillard — Wed, 13 Mar 2013 22:32:53 GMT

Agent E. coli mecanics: Fundamental elements of domestic chemical warfare

←Older revision		Revision as of 22:32, 13 March 2013
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	* Synthetic biology and reverse/forward DNA transcription "circuits"		* Synthetic biology and reverse/forward DNA transcription "circuits"
-	** luxR: Introduction to the Phosphohistidine ~~transferase genetic switch~~	+	** luxR: Introduction to the Phosphohistidine N-methyltransferase riboswitch: [[User:Etienne_Robillard/Notebook/30C6HSL\|a type of RNA transducing Mitogen Activated Protein Kinase (MAPK) using an imine as the drug carrier]]. (a serine-threonine specific kinase) [http://www.uniprot.org/uniprot/Q9NRX4 1]
	** [http://openwetware.org/index.php?title=User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent Agent Ecoli:ToggleSwitchComponent] (TL phage lambda)		** [http://openwetware.org/index.php?title=User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent Agent Ecoli:ToggleSwitchComponent] (TL phage lambda)

Etienne Robillard: add link to Arylcyclohexylamines notebook

Etienne Robillard — Wed, 13 Mar 2013 21:51:17 GMT

add link to Arylcyclohexylamines notebook

←Older revision		Revision as of 21:51, 13 March 2013
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	= Agent Ecoli: The whys and wherefores of Angel Dust is in the product. =		= Agent Ecoli: The whys and wherefores of Angel Dust is in the product. =
		+
		+	'''Notice: This page is outdated - For updates about Agent Ecoli and documentation about 1,2,3,4-tetrahydroisoquinolines derivatives, see [[User:Etienne_Robillard/Notebook/Arylcyclohexylamines\|Arylcyclohexylamines]].'''

	__TOC__		__TOC__

Etienne Robillard: /* Agent Ecoli: The whys and wherefores of Angel Dust is in the product. */

Etienne Robillard — Fri, 11 Jan 2013 13:08:40 GMT

Agent Ecoli: The whys and wherefores of Angel Dust is in the product.

←Older revision		Revision as of 13:08, 11 January 2013
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	[TODO]		[TODO]

-	== ~~Chapters~~ ==	+	== Concepts ==

	=== Introduction to Agent E. coli ===		=== Introduction to Agent E. coli ===

Etienne Robillard at 03:36, 1 December 2012

Etienne Robillard — Sat, 01 Dec 2012 03:36:38 GMT

←Older revision		Revision as of 03:36, 1 December 2012
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	=== summary ===		=== summary ===

-	~~Untested Agent E.coli 1.0 recipe:~~	+	Keywords: DNA methylation, cDNA, deoxyoligonucleotides, ribosomes, phosphatase (PAP2-like), Phosphohistidine regulation, Agent Dust, Agent Wet, PCP, Benzoyl, PHP, PC, lysogens, lytic cycle, trojan, horse, epygenetics, elephants, E. coli, K-12, MG1655, Agent Scooby Doo, aspartic acid (NMDA receptor)
-		+
-	* pick at least a combination of 1 (one) nerve agent (ie Benzoyl);	+
-	* autoclave custom plasmid(s) with ape... ;	+
-	* click on '''"I UNDERSTAND AND ACKNOWLEDGE THE RISKS OF CLONING FLUORESCENT ELEPHANTS, JUST GIMME SOME HARDCORE PLASMIDS TO MAKE MY CUSTOM OLIGOS!";	+
-	* add water (wash in bath with sodium hydrochloride solution..);	+
-	* To make a Lysis buffer reagent, add 1 (one) piperidine-hydrochloride derived reagent (ie Tris-HCl);	+
-	* To add a good source of aceton to your DNA mix, use 1 (one) 2-cyanoethyl derived reagent (acetonitrile);	+
-	* For doing oligos version, include 1 (one) organophosphate-based organic phosphatase compound (PHP, P-N, PC) as [http://www.organic-chemistry.org/protectivegroups/amino/boc-amino.htm base-labile] protection group...	+
-	* Moreover the DNA methylation process is believed to be regulated by endogenous enzymatic reactions implicated in phosphatase regulation.	+
-		+
-	Keywords: DNA methylation, cDNA, deoxyoligonucleotides, ribosomes, phosphatase (PAP2-like), Phosphohistidine regulation, Agent Dust, Agent Wet, PCP, Benzoyl, PHP, PC, lysogens, lytic cycle, trojan, horse, epygenetics, elephants, E. coli, K-12, MG1655, Agent Scooby Doo	+

	== Introduction ==		== Introduction ==

Etienne Robillard: /* Agent Ecoli: The whys and wherefores of Angel Dust is in the product. */

Etienne Robillard — Sat, 01 Dec 2012 03:13:12 GMT

Agent Ecoli: The whys and wherefores of Angel Dust is in the product.

←Older revision		Revision as of 03:13, 1 December 2012
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	== Introduction ==		== Introduction ==
		+
		+	* [http://www.openwetware.org/wiki/User:Etienne_Robillard/Notebook/Agent_Ecoli:Introduction A beginner introduction to Agent E. coli (Start here)]
		+

	[TODO]		[TODO]

Etienne Robillard: /* summary */

Etienne Robillard — Tue, 27 Nov 2012 17:45:25 GMT

summary

←Older revision		Revision as of 17:45, 27 November 2012
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	* add water (wash in bath with sodium hydrochloride solution..);		* add water (wash in bath with sodium hydrochloride solution..);
	* To make a Lysis buffer reagent, add 1 (one) piperidine-hydrochloride derived reagent (ie Tris-HCl);		* To make a Lysis buffer reagent, add 1 (one) piperidine-hydrochloride derived reagent (ie Tris-HCl);
-	* To add a good source of aceton to your DNA mix use 1 (one) 2-cyanoethyl derived reagent (acetonitrile);	+	* To add a good source of aceton to your DNA mix, use 1 (one) 2-cyanoethyl derived reagent (acetonitrile);
-	* For doing oligos version include 1 (one) organophosphate-based organic phosphatase compound (PHP, P-N, PC) as protection group...	+	* For doing oligos version, include 1 (one) organophosphate-based organic phosphatase compound (PHP, P-N, PC) as [http://www.organic-chemistry.org/protectivegroups/amino/boc-amino.htm base-labile] protection group...
	* Moreover the DNA methylation process is believed to be regulated by endogenous enzymatic reactions implicated in phosphatase regulation.		* Moreover the DNA methylation process is believed to be regulated by endogenous enzymatic reactions implicated in phosphatase regulation.