User:Etienne Robillard/Notebook/Agent Ecoli:PhosphohistidineTransferaseComponent

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In this experiment the goal is to construct a [http://www.bio-rad.com/prd/fr/FR/LSE/PDP/18721652-4f03-4c64-90f8-ab309e058dbb/Forensic-DNA-Fingerprinting-Kit forensic DNA fingerprinting kit] to verify the DNA fragments for evidences of electrocompetent-inducible promoters and cross-species gene vectors to produce bioluminiscence agaisnt a control group (MG1655).
In this experiment the goal is to construct a [http://www.bio-rad.com/prd/fr/FR/LSE/PDP/18721652-4f03-4c64-90f8-ab309e058dbb/Forensic-DNA-Fingerprinting-Kit forensic DNA fingerprinting kit] to verify the DNA fragments for evidences of electrocompetent-inducible promoters and cross-species gene vectors to produce bioluminiscence agaisnt a control group (MG1655).
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==== Experiment checklist ====
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* Control group will need to be plasmid-free - Obtain E. coli strain mg1655 as reference model for the electrophoresis experiment.
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* DNA extraction: data interpretation is subject to errors in the protocol - Find a coherent protocol specific for E.coli transformations using multi-plasmid vectors to transform E.coli into the desired mutant strain. 
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* familiarize yourself with the various electrophoresis assay methods - An important resource to learn and study the basics of E.coli transcription ab initio using recombinant DNA.   
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* more checklist fine points here... 
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==== See also ====
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* [http://openwetware.org/wiki/E._coli_restriction-modification_system E. coli restriction-modification system]

Revision as of 17:13, 31 October 2012

Contents

Agent Ecoli:Phosphohistidine Transferase Compound

OmpR and LuxR multi-step catalytic gene repression systems: Detection and forensic characterization of recombinant (synthetic) DNA material in vivo using dual-input, reversible enzymatic promoters

  • Reversion and repression of low to medium copy synthetic DNA plasmids (pSB3K3) using proteogenic amino acids and milk as organocatalysts DNA vectors for multi-step, combinatorial gene repression/knockout (KO) recipes for DNA origami forensic detection (N-P bound phosphoramidate), and exogenous drugs or antiviral proteins expressions with high bioluminiscence activity:
    • [Describe here a protocol or method for LuxR/EnvZ dual-input gene knockout (KO) detection and forensic analysis of antiviral phosphoramidate-conjugated compounds with Nitrogen bound molecules known as DNA acid...Include examples for OmpR::Phosphotransferase::Histidine multi-step vector expression in F- repressed colonies in the original/repressed state (control mg 1655 colonies), and finally perform electrophoresis for detection of electrocompetent colonies. In the event of plasmid-free control groups, it is expected that the repression rate is equal to the DNA polymerase transcription rate (but this has not been verified yet...) in luxR repression.]
    • Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this page for references.

Experiment

In this experiment the goal is to construct a forensic DNA fingerprinting kit to verify the DNA fragments for evidences of electrocompetent-inducible promoters and cross-species gene vectors to produce bioluminiscence agaisnt a control group (MG1655).

Experiment checklist

  • Control group will need to be plasmid-free - Obtain E. coli strain mg1655 as reference model for the electrophoresis experiment.
  • DNA extraction: data interpretation is subject to errors in the protocol - Find a coherent protocol specific for E.coli transformations using multi-plasmid vectors to transform E.coli into the desired mutant strain.
  • familiarize yourself with the various electrophoresis assay methods - An important resource to learn and study the basics of E.coli transcription ab initio using recombinant DNA.
  • more checklist fine points here...

See also

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