User:Etienne Robillard/Notebook/Agent Ecoli:PhosphohistidineTransferaseComponent

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== Agent Ecoli:Phosphohistidine Transferase Compound ==
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== Agent Ecoli:Phosphohistidine Transferase Component ==
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=== OmpR and LuxR multi-step catalytic gene repression systems: Detection and forensic characterization of recombinant (synthetic) DNA material in vivo using dual-input, reversible enzymatic promoters ===
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=== OmpR and LuxR ===
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* Reversion and repression of low to medium copy synthetic DNA plasmids (pSB3K3) using proteogenic amino acids and milk as organocatalysts DNA vectors for multi-step, combinatorial gene repression/knockout (KO) recipes for DNA origami forensic detection (N-P bound phosphoramidate), and exogenous drugs or antiviral proteins expressions with high bioluminiscence activity: 
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* Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this [http://2012.igem.org/Team:TU_Munich/Project/Caffeine page] for references.
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* In DNA/Purine deamination
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** Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this [http://2012.igem.org/Team:TU_Munich/Project/Caffeine page] for references.
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** http://www.kegg.jp/dbget-bin/www_bget?ko:K06223 (adenine-specific)
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== See also ==
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=== Experiment ===
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In this experiment the goal is to construct a [http://www.bio-rad.com/prd/fr/FR/LSE/PDP/18721652-4f03-4c64-90f8-ab309e058dbb/Forensic-DNA-Fingerprinting-Kit forensic DNA fingerprinting kit] to verify the DNA fragments for evidences of electrocompetent-inducible promoters and cross-species gene vectors to produce bioluminiscence agaisnt a control group (MG1655).
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==== Experiment checklist ====
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* Control group will need to be plasmid-free - Obtain E. coli strain mg1655 as reference model for the electrophoresis experiment.
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* DNA extraction: data interpretation is subject to errors in the protocol - Find a coherent protocol specific for E.coli transformations using multi-plasmid vectors to transform E.coli into the desired mutant strain. 
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* familiarize yourself with the various electrophoresis assay methods - An important resource to learn and study the basics of E.coli transcription ab initio using recombinant DNA.   
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* more checklist fine points here... 
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==== See also ====
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* [http://openwetware.org/wiki/E._coli_restriction-modification_system E. coli restriction-modification system]
* [http://openwetware.org/wiki/E._coli_restriction-modification_system E. coli restriction-modification system]
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* Synthetic ATP recombinant phosphonates conjugates: phosphoramidate, ANP, cyclic nucleotide phosphodiesterase (CNP), cAMP 
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* [http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks Standard E. coli Strain for BioBricks] - and this [http://www.freepatentsonline.com/y2003/0138937.html paper]
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* '''Osmotic regulation of the EnvZ gene :''' A E. coli switch for sensory autophosphorylation of the histidine kinase ?
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* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome]  - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]  
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* '''Light-induced drug delivery'''
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* [http://openwetware.org/wiki/Beta-galactosidase_assay Beta-galactosidase assay] - EC 2.7.13.3
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* http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG12414&redirect=T
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* [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt sequence for M13k07 reengineered plasmid] : '''Note''' : If pMR101 is a gene vector derived from pACYC177 then clearly the resulting [http://www.uic.edu/classes/phar/phar331/lecture6/ plasmid cloning vector] will be a '''synthetic construct''' derived from a M13 like bacteriophage, thus the use of restriction enzymes for acquiring inter-species genes (OmpR/EnvZ) is likely a positive indicator of a scheme to induce '''horizontal''' gene regulation using bacterial conjugation like sex in elephants and the ''E. coli'' K-12 MG1655 strain.
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* [http://openwetware.org/wiki/Beta-galactosidase_assay Beta-galactosidase assay]
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* EC 2.7.13.3
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* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome]  - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]
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* '''The Matrix (1999)'''
* '''The Matrix (1999)'''

Current revision

Contents

Agent Ecoli:Phosphohistidine Transferase Component

OmpR and LuxR

  • Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this page for references.
  • In DNA/Purine deamination

See also

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