User:Etienne Robillard/Notebook/Agent Ecoli:PhosphohistidineTransferaseComponent

From OpenWetWare

< User:Etienne Robillard | Notebook(Difference between revisions)
Jump to: navigation, search
m (OmpR and LuxR multi-step catalytic gene repression systems: Detection and forensic characterization of recombinant (synthetic) DNA material in vivo using dual-input, reversible enzymatic promoters)
Current revision (09:55, 8 August 2013) (view source)
 
(2 intermediate revisions not shown.)
Line 1: Line 1:
__TOC__
__TOC__
-
== Agent Ecoli:Phosphohistidine Transferase Compound ==
+
== Agent Ecoli:Phosphohistidine Transferase Component ==
=== OmpR and LuxR ===
=== OmpR and LuxR ===
-
** Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this [http://2012.igem.org/Team:TU_Munich/Project/Caffeine page] for references.
+
* Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this [http://2012.igem.org/Team:TU_Munich/Project/Caffeine page] for references.
-
 
+
* In DNA/Purine deamination
-
=== Experiment ===
+
** http://www.kegg.jp/dbget-bin/www_bget?ko:K06223 (adenine-specific)
-
 
+
-
In this experiment the goal is to construct a [http://www.bio-rad.com/prd/fr/FR/LSE/PDP/18721652-4f03-4c64-90f8-ab309e058dbb/Forensic-DNA-Fingerprinting-Kit forensic DNA fingerprinting kit] to verify the DNA fragments for evidences of electrocompetent-inducible promoters and cross-species gene vectors to produce bioluminiscence agaisnt a control group (MG1655).
+
-
 
+
-
==== Experiment checklist ====
+
-
 
+
-
* Control group will need to be plasmid-free - Obtain E. coli strain mg1655 as reference model for the electrophoresis experiment.
+
-
* DNA extraction: data interpretation is subject to errors in the protocol - Find a coherent protocol specific for E.coli transformations using multi-plasmid vectors to transform E.coli into the desired mutant strain. 
+
-
* familiarize yourself with the various electrophoresis assay methods - An important resource to learn and study the basics of E.coli transcription ab initio using recombinant DNA.   
+
-
* more checklist fine points here... 
+
-
 
+
== See also ==
== See also ==

Current revision

Contents

Agent Ecoli:Phosphohistidine Transferase Component

OmpR and LuxR

  • Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this page for references.
  • In DNA/Purine deamination

See also

Personal tools