User:Etienne Robillard/Notebook/Agent Ecoli:Plasmid Strains carrying laclq mutation

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(BW26434)
(BW26434)
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* pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid])
* pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid])
* accept inserts up to ~30 to 45 kb [http://xnet.rrc.mb.ca/davidb/cloning_vectors.htm 1].
* accept inserts up to ~30 to 45 kb [http://xnet.rrc.mb.ca/davidb/cloning_vectors.htm 1].
 +
* is is that hard to think without someone to tell you what to think?

Revision as of 14:32, 5 December 2012

Contents

Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli

Disclaimer

WOOP WOOP!

Hypothesis

  • GCG CAA ? [1]

D1210 perhaps just doesn't fit...

  • I need a "non-leaky" strain which express the araBAD mutation.
    • lambda lysogen (ie HB101)
    • F+ plasmid
    • lacY
    • hsdR restriction system (ie: leaky restriction system)
    • NO appropriate mutation of ARA operon/transport ! WOOP WOOP

BW26434

  • Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
  • However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.
    • Are they doing this as a habit or training to become excessively insane?
    • A Cloning ninja signature perhaps ?
    • Random muslings ?
    •  ?
    • WOOP WOOP !
  • pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a cos mid)
  • accept inserts up to ~30 to 45 kb 1.
  • is is that hard to think without someone to tell you what to think?
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