User:Etienne Robillard/Notebook/Agent Ecoli:SolidPhasePeptideSynthesisReagentEvidencesInAgentScoobyDoo

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(Expected results)
Current revision (07:39, 23 August 2015) (view source)
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== Measuring the amplitude of the synthetic reagent lysis TE buffer on NMDA receptor ==
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* Date: 2012/12/03 10:29 AM EDT 
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* Organism: E. coli (K-12 substr.)
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* Strain: MG1655(?)
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=== Hypothesis ===
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Amine protection groups (piperidine based) undergo chiral nucleophilic substitution on Histidine based autophosphorylation.
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=== Experiment ===
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==== Material ====
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* 1 x 10ml TE = TAE -> 10x buffer (Tris-HCl) (see [TE]
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==== Expected results ====
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* '''Amine''' protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation)
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* a heterocyclic base-labile group (NHC?)
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* [[IPTG]] inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)
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** in that case a matching E. coli genotype is "HB101 lacIq, lacY+" (ie [http://partsregistry.org/Part:BBa_V1003 laclq])
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** From [http://www.sigmaaldrich.com/life-science/molecular-biology/cloning-and-expression/learning-center/genotypes-of-e-coli.html Sigma], the expected genotype matching the laclq strain is:
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*** HB101 F– Δ(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13h
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==== References ====
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* [https://www.openwetware.org/wiki/TE TE]
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* [http://cgsc.biology.yale.edu/Strain.php?ID=9130 AB266]
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* [http://cgsc.biology.yale.edu/Strain.php?ID=11958 E.coli B]: WOOP WOOP
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