User:Etienne Robillard/Notebook/Agent Ecoli:SolidPhasePeptideSynthesisReagentEvidencesInAgentScoobyDoo

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(Measuring the amplitude of the synthetic reagent lysis TE buffer on NMDA receptor)
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* Date: 2012/12/03 10:29 AM EDT   
* Date: 2012/12/03 10:29 AM EDT   
* Organism: E. coli (K-12 substr.)
* Organism: E. coli (K-12 substr.)
-
* Strain: MG1655(?)
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* Strain: Probably D1210, a HB101/K-12 hybrid strain which overexpress the Lacl repressor.
 +
 
=== Hypothesis ===
=== Hypothesis ===
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* '''Amine''' protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation)
* '''Amine''' protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation)
* a heterocyclic base-labile group (NHC?)
* a heterocyclic base-labile group (NHC?)
 +
* [[IPTG]] inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)
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** in that case a matching E. coli genotype is "HB101 lacIq, lacY+" (ie [http://partsregistry.org/Part:BBa_V1003 laclq])
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** From [http://www.sigmaaldrich.com/life-science/molecular-biology/cloning-and-expression/learning-center/genotypes-of-e-coli.html Sigma], the expected genotype matching the laclq strain is:
 +
*** HB101 F– Δ(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13h
==== References ====
==== References ====
* [https://www.openwetware.org/wiki/TE TE]
* [https://www.openwetware.org/wiki/TE TE]
 +
* [http://cgsc.biology.yale.edu/Strain.php?ID=9130 AB266]
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* [http://cgsc.biology.yale.edu/Strain.php?ID=11958 E.coli B]: WOOP WOOP
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* [[IPTG]]

Revision as of 20:57, 4 December 2012

Contents

Measuring the amplitude of the synthetic reagent lysis TE buffer on NMDA receptor

  • Date: 2012/12/03 10:29 AM EDT
  • Organism: E. coli (K-12 substr.)
  • Strain: Probably D1210, a HB101/K-12 hybrid strain which overexpress the Lacl repressor.


Hypothesis

Amine protection groups (piperidine based) undergo chiral nucleophilic substitution on Histidine based autophosphorylation.

Experiment

Material

  • 1 x 10ml TE = TAE -> 10x buffer (Tris-HCl) (see [TE]

Expected results

  • Amine protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation)
  • a heterocyclic base-labile group (NHC?)
  • IPTG inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)
    • in that case a matching E. coli genotype is "HB101 lacIq, lacY+" (ie laclq)
    • From Sigma, the expected genotype matching the laclq strain is:
      • HB101 F– Δ(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13h

References

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