User:Etienne Robillard/Notebook/Agent Ecoli:SolidPhasePeptideSynthesisReagentEvidencesInAgentScoobyDoo

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(Hypothesis)
Current revision (20:01, 12 December 2012) (view source)
(Hypothesis)
 
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* artificial cos-mid size is 30-45 bp length => reduced genome ?
* artificial cos-mid size is 30-45 bp length => reduced genome ?
* The NMDA receptor is voltage gated: [http://www.physicsforums.com/showthread.php?t=632781 The addition of EDTA (mg2+) ions to the reaction may act as '''NMDA receptor antagonist''' by binding to the NMDA ligand...]
* The NMDA receptor is voltage gated: [http://www.physicsforums.com/showthread.php?t=632781 The addition of EDTA (mg2+) ions to the reaction may act as '''NMDA receptor antagonist''' by binding to the NMDA ligand...]
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* [[IPTG]] induction is a chemical reaction which may be neutralized with 1mL NaHC03... (Sodium bicarbonate, 2.99$) [[http://en.wikipedia.org/wiki/Sodium_bicarbonate#Neutralization_of_acids_and_bases 1]] [[http://bioserv.fiu.edu/~biolab/labs/genetics/lac%20operon.htm 2]] [[http://www.openwetware.org/wiki/BE.109:Protein_engineering/Assessing_beta-galactosidase 3]] [[http://www.openwetware.org/wiki/Beta-Galactosidase_Assay_(A_better_Miller) 4]]
=== Experiment ===
=== Experiment ===
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* [http://cgsc.biology.yale.edu/Strain.php?ID=11958 E.coli B]: WOOP WOOP
* [http://cgsc.biology.yale.edu/Strain.php?ID=11958 E.coli B]: WOOP WOOP
* [[IPTG]]
* [[IPTG]]
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* [[EDTA]]

Current revision

Contents

Measuring the amplitude of the synthetic reagent lysis TE buffer on NMDA receptor

  • Date: 2012/12/03 10:29 AM EDT
  • Organism: E. coli (K-12 substr.)
  • Strain: BW26434


Hypothesis

  • Amine protection groups (piperidine based) undergo chiral nucleophilic substitution on Histidine based autophosphorylation.
  • Cosmid like recombinase system for -35 lacl+ (LacZ) transcription, thus no infection produced; (no lacY)
  • orthogonal oligo/peptide protection using methylamino (methyltransferase) agents;
  • methylamino buffering agents used as intermediates for orthogonal protection; (probably a side-effect of Magnesium chelation with EDTA?)
  • artificial cos-mid size is 30-45 bp length => reduced genome ?
  • The NMDA receptor is voltage gated: The addition of EDTA (mg2+) ions to the reaction may act as NMDA receptor antagonist by binding to the NMDA ligand...
  • IPTG induction is a chemical reaction which may be neutralized with 1mL NaHC03... (Sodium bicarbonate, 2.99$) [1] [2] [3] [4]

Experiment

Material

  • 1 x 10ml TE = TAE -> 10x buffer (Tris-HCl) (see [TE]

Expected results

  • Amine protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation)
  • a heterocyclic base-labile group (NHC?)
  • IPTG inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)
    • in that case a matching E. coli genotype is "HB101 lacIq, lacY+" (ie laclq)
    • From Sigma, the expected genotype matching the laclq strain is:
      • HB101 F– Δ(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13h
  • (2R)-2-(methylamino)succinic acid - An orthogonal N-methyltransferase/NH2 base labile compound; exogenous NMDA receptor antagonist compound (Ketamine-like); synthetically recombined oligonucleotide protection group (dA-dT) for solid-phase peptide synthesis (SPPS), etc (see also PHP, Succinic_acid, and BW26434.)
    • important: N-methyl-D-asparte receptor is a "sensory" neurotransmitter with analogous functions to the TRPV neurotransmitter but for brain functions including learning and memory.

References

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