User:Etienne Robillard/Notebook/Agent Ecoli:SolidPhasePeptideSynthesisReagentEvidencesInAgentScoobyDoo
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Revision as of 14:18, 4 December 2012
Contents |
Measuring the amplitude of the synthetic reagent lysis TE buffer on NMDA receptor
- Date: 2012/12/03 10:29 AM EDT
- Organism: E. coli (K-12 substr.)
- Strain: MG1655(?)
Hypothesis
Amine protection groups (piperidine based) undergo chiral nucleophilic substitution on Histidine based autophosphorylation.
Experiment
Material
- 1 x 10ml TE = TAE -> 10x buffer (Tris-HCl) (see [TE]
Expected results
- Amine protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation)
- a heterocyclic base-labile group (NHC?)
- IPTG inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)
- in that case a matching E. coli genotype is "HB101 lacIq, lacY+" (ie laclq)
