User:Etienne Robillard/Notebook/Agent Ecoli:SolidPhasePeptideSynthesisReagentEvidencesInAgentScoobyDoo
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* '''Amine''' protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation) | * '''Amine''' protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation) | ||
* a heterocyclic base-labile group (NHC?) | * a heterocyclic base-labile group (NHC?) | ||
| + | * [[IPTG]] inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!) | ||
==== References ==== | ==== References ==== | ||
* [https://www.openwetware.org/wiki/TE TE] | * [https://www.openwetware.org/wiki/TE TE] | ||
Revision as of 13:49, 4 December 2012
Contents |
Measuring the amplitude of the synthetic reagent lysis TE buffer on NMDA receptor
- Date: 2012/12/03 10:29 AM EDT
- Organism: E. coli (K-12 substr.)
- Strain: MG1655(?)
Hypothesis
Amine protection groups (piperidine based) undergo chiral nucleophilic substitution on Histidine based autophosphorylation.
Experiment
Material
- 1 x 10ml TE = TAE -> 10x buffer (Tris-HCl) (see [TE]
Expected results
- Amine protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation)
- a heterocyclic base-labile group (NHC?)
- IPTG inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)
