User:Etienne Robillard/Notebook/Agent Ecoli:SolidPhasePeptideSynthesisReagentEvidencesInAgentScoobyDoo
From OpenWetWare
(Difference between revisions)
(→Expected results) |
(→Measuring the amplitude of the synthetic reagent lysis TE buffer on NMDA receptor) |
||
| Line 3: | Line 3: | ||
* Date: 2012/12/03 10:29 AM EDT | * Date: 2012/12/03 10:29 AM EDT | ||
* Organism: E. coli (K-12 substr.) | * Organism: E. coli (K-12 substr.) | ||
| - | * Strain: | + | * Strain: Probably D1210, a HB101/K-12 hybrid strain which overexpress the Lacl repressor. |
| + | |||
=== Hypothesis === | === Hypothesis === | ||
| Line 29: | Line 30: | ||
* [http://cgsc.biology.yale.edu/Strain.php?ID=9130 AB266] | * [http://cgsc.biology.yale.edu/Strain.php?ID=9130 AB266] | ||
* [http://cgsc.biology.yale.edu/Strain.php?ID=11958 E.coli B]: WOOP WOOP | * [http://cgsc.biology.yale.edu/Strain.php?ID=11958 E.coli B]: WOOP WOOP | ||
| + | * [[IPTG]] | ||
Revision as of 20:57, 4 December 2012
Contents |
Measuring the amplitude of the synthetic reagent lysis TE buffer on NMDA receptor
- Date: 2012/12/03 10:29 AM EDT
- Organism: E. coli (K-12 substr.)
- Strain: Probably D1210, a HB101/K-12 hybrid strain which overexpress the Lacl repressor.
Hypothesis
Amine protection groups (piperidine based) undergo chiral nucleophilic substitution on Histidine based autophosphorylation.
Experiment
Material
- 1 x 10ml TE = TAE -> 10x buffer (Tris-HCl) (see [TE]
Expected results
- Amine protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation)
- a heterocyclic base-labile group (NHC?)
- IPTG inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)
