User:Etienne Robillard/Notebook/Agent Ecoli:SolidPhasePeptideSynthesisReagentEvidencesInAgentScoobyDoo

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Contents

Measuring the amplitude of the synthetic reagent lysis TE buffer on NMDA receptor

  • Date: 2012/12/03 10:29 AM EDT
  • Organism: E. coli (K-12 substr.)
  • Strain: MG1655(?)

Hypothesis

Amine protection groups (piperidine based) undergo chiral nucleophilic substitution on Histidine based autophosphorylation.

Experiment

Material

  • 1 x 10ml TE = TAE -> 10x buffer (Tris-HCl) (see [TE]

Expected results

  • Amine protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation)
  • a heterocyclic base-labile group (NHC?)
  • IPTG inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)
    • in that case a matching E. coli genotype is "HB101 lacIq, lacY+" (ie laclq)
    • From Sigma, the expected genotype matching the laclq strain is:
      • HB101 F– Δ(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13h

References

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