User:Etienne Robillard/Notebook/Agent Ecoli:Plasmid Strains carrying laclq mutation

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(New page: == Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli == == Disclaimer == WOOP WOOP! == Hypothesis == * GCG CAA ? [[http://cgsc.biology.yal...)
Current revision (11:42, 31 January 2015) (view source)
 
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== Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli ==
== Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli ==
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* GCG CAA ? [[http://cgsc.biology.yale.edu/Strain.php?ID=65553 1]]
* GCG CAA ? [[http://cgsc.biology.yale.edu/Strain.php?ID=65553 1]]
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=== D1210 perhaps just doesn't fit... ===
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* I need a "non-leaky" strain which express the  [http://cgsc.biology.yale.edu/Mutation.php?ID=64657 araBAD] mutation.
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** lambda lysogen (ie HB101)
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** F+ plasmid
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** lacY
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** EGFR-hsdR restriction system (ie: a purely leaky restriction system)
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** NO appropriate mutation of ARA operon/transport ! WOOP WOOP
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=== Biobrick x BW26434 ===
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* Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
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* pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid])
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* accept inserts up to ~30 to 45 kb [http://xnet.rrc.mb.ca/davidb/cloning_vectors.htm 1]
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* Also see [[Phosphoramidate]].

Current revision

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Contents

Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli

Disclaimer

WOOP WOOP!

Hypothesis

  • GCG CAA ? [1]

D1210 perhaps just doesn't fit...

  • I need a "non-leaky" strain which express the araBAD mutation.
    • lambda lysogen (ie HB101)
    • F+ plasmid
    • lacY
    • EGFR-hsdR restriction system (ie: a purely leaky restriction system)
    • NO appropriate mutation of ARA operon/transport ! WOOP WOOP

Biobrick x BW26434

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