User:Etienne Robillard/Notebook/Agent Ecoli:Strains carrying laclq mutation
From OpenWetWare
(Difference between revisions)
(→D1210 perhaps just doesn't fit...) |
|||
| Line 11: | Line 11: | ||
=== D1210 perhaps just doesn't fit... === | === D1210 perhaps just doesn't fit... === | ||
| - | + | * I need a "non-leaky" strain which express the [http://cgsc.biology.yale.edu/Mutation.php?ID=64657 araBAD] mutation. | |
** lambda lysogen (ie HB101) | ** lambda lysogen (ie HB101) | ||
** F+ plasmid | ** F+ plasmid | ||
Revision as of 23:05, 4 December 2012
Contents |
Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli
Disclaimer
WOOP WOOP!
Hypothesis
- GCG CAA ? [1]
D1210 perhaps just doesn't fit...
- I need a "non-leaky" strain which express the araBAD mutation.
- lambda lysogen (ie HB101)
- F+ plasmid
- lacY
- hsdR restriction system (ie: leaky restriction system)
- NO appropriate mutation of ARA operon/transport ! WOOP WOOP
BW26434
- Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
- However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.
- Are they doing this as a habit or training to become excessively insane?
- A Cloning ninja signature perhaps ?
- Random muslings ?
- ?
- WOOP WOOP !
