User:Etienne Robillard/Notebook/Agent Ecoli:Plasmid Strains carrying laclq mutation

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Current revision (11:42, 31 January 2015) (view source)
 
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== Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli ==
== Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli ==
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=== D1210 perhaps just doesn't fit... ===
=== D1210 perhaps just doesn't fit... ===
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** I need a "non-leaky" strain which express the  [http://cgsc.biology.yale.edu/Mutation.php?ID=64657 araBAD] mutation.
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* I need a "non-leaky" strain which express the  [http://cgsc.biology.yale.edu/Mutation.php?ID=64657 araBAD] mutation.
** lambda lysogen (ie HB101)
** lambda lysogen (ie HB101)
** F+ plasmid  
** F+ plasmid  
** lacY
** lacY
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** hsdR restriction system (ie: leaky restriction system)
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** EGFR-hsdR restriction system (ie: a purely leaky restriction system)
** NO appropriate mutation of ARA operon/transport ! WOOP WOOP
** NO appropriate mutation of ARA operon/transport ! WOOP WOOP
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=== BW26434 ===
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=== Biobrick x BW26434 ===
* Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
* Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
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* However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.  
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* pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid])
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** Are they doing this as a habit or training to become excessively insane? 
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* accept inserts up to ~30 to 45 kb [http://xnet.rrc.mb.ca/davidb/cloning_vectors.htm 1]
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** A Cloning ninja signature perhaps ?
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* Also see [[Phosphoramidate]].
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** Random muslings ?
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** ?
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** WOOP WOOP !
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Current revision

It has been requested that this page be removed with restriction endonucleases.
Other articles for deletion are listed here.


Contents

Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli

Disclaimer

WOOP WOOP!

Hypothesis

  • GCG CAA ? [1]

D1210 perhaps just doesn't fit...

  • I need a "non-leaky" strain which express the araBAD mutation.
    • lambda lysogen (ie HB101)
    • F+ plasmid
    • lacY
    • EGFR-hsdR restriction system (ie: a purely leaky restriction system)
    • NO appropriate mutation of ARA operon/transport ! WOOP WOOP

Biobrick x BW26434

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