User:Etienne Robillard/Notebook/Agent Ecoli:Plasmid Strains carrying laclq mutation

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(D1210 perhaps just doesn't fit...)
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=== D1210 perhaps just doesn't fit... ===
=== D1210 perhaps just doesn't fit... ===
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** I need a "non-leaky" strain which express the  [http://cgsc.biology.yale.edu/Mutation.php?ID=64657 araBAD] mutation.
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* I need a "non-leaky" strain which express the  [http://cgsc.biology.yale.edu/Mutation.php?ID=64657 araBAD] mutation.
** lambda lysogen (ie HB101)
** lambda lysogen (ie HB101)
** F+ plasmid  
** F+ plasmid  

Revision as of 23:05, 4 December 2012

Contents

Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli

Disclaimer

WOOP WOOP!

Hypothesis

  • GCG CAA ? [1]

D1210 perhaps just doesn't fit...

  • I need a "non-leaky" strain which express the araBAD mutation.
    • lambda lysogen (ie HB101)
    • F+ plasmid
    • lacY
    • hsdR restriction system (ie: leaky restriction system)
    • NO appropriate mutation of ARA operon/transport ! WOOP WOOP

BW26434

  • Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
  • However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.
    • Are they doing this as a habit or training to become excessively insane?
    • A Cloning ninja signature perhaps ?
    • Random muslings ?
    •  ?
    • WOOP WOOP !
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