User:Etienne Robillard/Notebook/Agent Ecoli:Strains carrying laclq mutation
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(New page: == Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli == == Disclaimer == WOOP WOOP! == Hypothesis == * GCG CAA ? [[http://cgsc.biology.yal...) |
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* GCG CAA ? [[http://cgsc.biology.yale.edu/Strain.php?ID=65553 1]] | * GCG CAA ? [[http://cgsc.biology.yale.edu/Strain.php?ID=65553 1]] | ||
| + | |||
| + | === D1210 perhaps just doesn't fit... === | ||
| + | |||
| + | ** I need a "non-leaky" strain which express the [http://cgsc.biology.yale.edu/Mutation.php?ID=64657 araBAD] mutation. | ||
| + | ** lambda lysogen (ie HB101) | ||
| + | ** F+ plasmid | ||
| + | ** lacY | ||
| + | ** hsdR restriction system (ie: leaky restriction system) | ||
| + | ** NO appropriate mutation of ARA operon/transport ! WOOP WOOP | ||
| + | |||
| + | === BW26434 === | ||
| + | |||
| + | * Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks | ||
| + | * However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking. | ||
| + | ** Are they doing this as a habit or training to become excessively insane? | ||
| + | ** A Cloning ninja signature perhaps ? | ||
| + | ** Random muslings ? | ||
| + | ** ? | ||
| + | ** WOOP WOOP ! | ||
Revision as of 23:00, 4 December 2012
Contents |
Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli
Disclaimer
WOOP WOOP!
Hypothesis
- GCG CAA ? [1]
D1210 perhaps just doesn't fit...
- I need a "non-leaky" strain which express the araBAD mutation.
- lambda lysogen (ie HB101)
- F+ plasmid
- lacY
- hsdR restriction system (ie: leaky restriction system)
- NO appropriate mutation of ARA operon/transport ! WOOP WOOP
BW26434
- Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
- However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.
- Are they doing this as a habit or training to become excessively insane?
- A Cloning ninja signature perhaps ?
- Random muslings ?
- ?
- WOOP WOOP !
