User:Etienne Robillard/Notebook/Chemtrails911 notebook/Agent Ecoli:PhosphohistidineTransferaseComponent

From OpenWetWare

< User:Etienne Robillard | Notebook | Chemtrails911 notebook(Difference between revisions)
Jump to: navigation, search
(→See also)
Current revision (04:52, 7 November 2012) (view source)
(→Agent Ecoli:Phosphohistidine Transferase Compound)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
__TOC__
__TOC__
-
== Agent Ecoli:Phosphohistidine Transferase Compound ==
+
== Agent Ecoli:Phosphohistidine Transferase Component ==
-
=== OmpR and LuxR multi-step catalytic gene repression systems: Detection and forensic characterization of recombinant (synthetic) DNA material in vivo using dual-input, reversible enzymatic promoters ===
+
=== OmpR and LuxR ===
-
* Reversion and repression of low to medium copy synthetic DNA plasmids (pSB3K3) using proteogenic amino acids and milk as organocatalysts DNA vectors for multi-step, combinatorial gene repression/knockout (KO) recipes for DNA origami forensic detection (N-P bound phosphoramidate), and exogenous drugs or antiviral proteins expressions with high bioluminiscence activity:
+
* Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this [http://2012.igem.org/Team:TU_Munich/Project/Caffeine page] for references.
-
** Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this [http://2012.igem.org/Team:TU_Munich/Project/Caffeine page] for references.
+
== See also ==
-
 
+
-
=== Experiment ===
+
-
 
+
-
In this experiment the goal is to construct a [http://www.bio-rad.com/prd/fr/FR/LSE/PDP/18721652-4f03-4c64-90f8-ab309e058dbb/Forensic-DNA-Fingerprinting-Kit forensic DNA fingerprinting kit] to verify the DNA fragments for evidences of electrocompetent-inducible promoters and cross-species gene vectors to produce bioluminiscence agaisnt a control group (MG1655).
+
-
 
+
-
==== Experiment checklist ====
+
-
 
+
-
* Control group will need to be plasmid-free - Obtain E. coli strain mg1655 as reference model for the electrophoresis experiment.
+
-
* DNA extraction: data interpretation is subject to errors in the protocol - Find a coherent protocol specific for E.coli transformations using multi-plasmid vectors to transform E.coli into the desired mutant strain. 
+
-
* familiarize yourself with the various electrophoresis assay methods - An important resource to learn and study the basics of E.coli transcription ab initio using recombinant DNA.   
+
-
* more checklist fine points here... 
+
-
 
+
-
==== See also ====
+
* [http://openwetware.org/wiki/E._coli_restriction-modification_system E. coli restriction-modification system]
* [http://openwetware.org/wiki/E._coli_restriction-modification_system E. coli restriction-modification system]
-
* Synthetic ATP recombinant phosphonates conjugates: phosphoramidate, ANP, cyclic nucleotide phosphodiesterase (CNP), cAMP 
+
* [http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks Standard E. coli Strain for BioBricks] - and this [http://www.freepatentsonline.com/y2003/0138937.html paper]
-
* '''Osmotic regulation of the EnvZ gene :''' A E. coli switch for sensory autophosphorylation of the histidine kinase ?
+
* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome]  - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]
-
* '''Light-induced drug delivery'''
+
* [http://openwetware.org/wiki/Beta-galactosidase_assay Beta-galactosidase assay] - EC 2.7.13.3
-
* http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG12414&redirect=T
+
* [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt sequence for M13k07 reengineered plasmid] : '''Note''' : If pMR101 is a gene vector derived from pACYC177 then clearly the resulting [http://www.uic.edu/classes/phar/phar331/lecture6/ plasmid cloning vector] will be a '''synthetic construct''' derived from a M13 like bacteriophage, thus the use of restriction enzymes for acquiring inter-species genes (OmpR/EnvZ) is likely a positive indicator of a scheme to induce '''horizontal''' gene regulation using bacterial conjugation like sex in elephants and the ''E. coli'' K-12 MG1655 strain.
-
* [http://openwetware.org/wiki/Beta-galactosidase_assay Beta-galactosidase assay]
+
-
* EC 2.7.13.3
+
* '''The Matrix (1999)'''
* '''The Matrix (1999)'''

Current revision

Contents

Agent Ecoli:Phosphohistidine Transferase Component

OmpR and LuxR

  • Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this page for references.

See also

Retrieved from "http://www.openwetware.org/wiki/User:Etienne_Robillard/Notebook/Chemtrails911_notebook/Agent_Ecoli:PhosphohistidineTransferaseComponent"
Personal tools