Revision as of 05:34, 9 November 2012

Plasmid cloning vectors for gene therapy with phosphohistidine-based oligos

cDNA:

N-6-benzoyl-deoxyadenosine phosphoramidite - Notice the P-N bound indicating the linkage of a phosphonate diester to a benzoyl atom :)
N-3-oxo-hexanoyl-L-homoserine lactone - A light-inducible phosphohistidine-bound AHL ? A(denosine)TP/cAMP dependent.
Reminder:
- NHC = (N heterocycle carbene) - Chemical compounds with a N chiral prodrug switch - highly enantioselective molecules
- N = indicates a Nitrogen atom
- P = a Phosphonate (inorganic state) - a compound for oligo synthesis using the nucleoside phosphoramidite method.
- P-N = phosphoramidate (a Nitrogen atom bound to a Phosphonate ester in nucleophilic substitution).

deoxynucleotide synthesis:

cDNA synthesis building block = deoxynucleoside + 3'-phosphonate = synthetic nucleotide analog (dATP) 1

oligodeoxyribonucleotide synthesis:

short 50-200(?) bp restriction endonucleases enzymes for cDNA catalysis/plasmid vector cloning in E. coli
building blocks for oligonucleotides assembly with phosphoramidites method 1
see also Oligonucleotide Synthesis

M13K07 cloning in E. coli:

@@ Line 17: / Line 17: @@
 oligodeoxyribonucleotide synthesis:
 * short 50-200(?) bp restriction endonucleases enzymes for cDNA catalysis/plasmid vector cloning in ''E. coli''
-* building blocks for oligonucleotides synthesis with phosphoramidites method [http://en.wikipedia.org/wiki/Oligonucleotide 1]
+* building blocks for oligonucleotides assembly with phosphoramidites method [http://en.wikipedia.org/wiki/Oligonucleotide 1]
 * see also [http://openwetware.org/wiki/CH391L/S12/GeneandGenomeSynthesis#Oligonucleotide_Synthesis Oligonucleotide Synthesis]