User:Etienne Robillard/Notebook/Chemtrails911 notebook/Agent Ecoli:Plasmid cloning vectors for gene therapy with phosphohistidine based oligos

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*** phospho'''tri'''ester = a '''tri'''cyclic N-derived nitrogenous base bound to a Histidine residue made for '''DNA''' synthesis.  
*** phospho'''tri'''ester = a '''tri'''cyclic N-derived nitrogenous base bound to a Histidine residue made for '''DNA''' synthesis.  
* '''Thus phosphohistidine based phosphoramidates can be classified as purine analogues; the substance (Purines) was formally discovered by Emil Fischer during WW1 as a fundamental group of heterocyclic amino acids.'''
* '''Thus phosphohistidine based phosphoramidates can be classified as purine analogues; the substance (Purines) was formally discovered by Emil Fischer during WW1 as a fundamental group of heterocyclic amino acids.'''
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** see also [http://en.wikipedia.org/wiki/Piperidine Piperidine] : "Piperidine is also commonly used in chemical degradation reactions, such as the sequencing of DNA in the cleavage of particular modified nucleotides. Piperidine is also commonly used as a base for the deprotection of Fmoc-amino acids used in '''[http://openwetware.org/wiki/Solid_Phase_Peptide_Synthesis solid-phase peptide synthesis].'''"
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** see Brink et al - Orthogonal translation/transcription and specialized ribosomes:  http://www.ncbi.nlm.nih.gov/pubmed/7758959
== References ==
== References ==

Revision as of 08:05, 22 March 2013

Plasmid cloning vectors for gene therapy with phosphohistidine-based oligos

Contents

Eukariotic DNA Promoters

cDNA:

  • N-6-benzoyl-deoxyadenosine phosphoramidite - Notice the P-N bound indicating the linkage of a phosphonate diester to a benzoyl atom :)
  • N-3-oxo-hexanoyl-L-homoserine lactone - A light-inducible phosphohistidine-bound AHL ? A(denosine)TP/cAMP dependent.
  • Reminder:
    • NHC = (N heterocycle carbene) - Chemical compounds with a N chiral prodrug switch - highly enantioselective molecules
    • N = indicates a Nitrogen atom
    • P = a Phosphonate (inorganic state) - a compound for oligo synthesis using the nucleoside phosphoramidite method.
    • P-N = phosphoramidate (a Nitrogen atom bound to a Phosphonate ester in nucleophilic substitution).

deoxynucleotide synthesis:

  • cDNA synthesis building block = deoxynucleoside + 3'-phosphonate = synthetic nucleotide analog (dATP) 1

oligodeoxyribonucleotide synthesis:

  • short 50-200(?) bp restriction endonucleases enzymes for cDNA catalysis/plasmid vector cloning in E. coli
  • building blocks for oligonucleotides assembly with phosphoramidites method 1
  • see also Oligonucleotide Synthesis
  • 1 mutation, 1 oligo assay (phosphoramidite-based oligo synthesis): [1]

M13K07 cloning in E. coli:

  • amino-acid sequence signature (Myc epitope tag) = EQKLISEEDL

Orthogonal DNA synthesis with Benzoyl-derived phosphotriester protecting groups (dA)

  • note! note! Important: Can you elucidate the reasoning of using an carboxilic acid as protecting group for DNA assembly?
    • FIRST PROBABLE PROTECTION GROUP (dA):
      • Bz = Benzoyl (Bz, benzoic acid ester) : 2nd step in the catalysis of Benzilic acid from oxidation of Benzoic acid. See "benzoic acid". A very common [reagent] for site-directed oligonucleotide synthesis.
      • Note: Consensus promoter sequences are by definition strong promoters!
    • 2ND HIGHLY PROBABLE PROTECTION GROUP (araBAD):
      • 2-cyanoethyl : for protecting phosphoramidites based nucleosides? XXX
      • araBAD XXX
      • phosphotriester = a tricyclic N-derived nitrogenous base bound to a Histidine residue made for DNA synthesis.
  • Thus phosphohistidine based phosphoramidates can be classified as purine analogues; the substance (Purines) was formally discovered by Emil Fischer during WW1 as a fundamental group of heterocyclic amino acids.

References

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