User:Etienne Robillard/Notebook/Chemtrails911 notebook/ForensicEvidencesChart

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=== Synopsis ===
=== Synopsis ===
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The goal of this project is to collect scientific evidences of state-sponsored collusion in the research and development of a precise technology known
 
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as a '''"genetic switch"''', or "transcriptional devices", ie well-designed biological systems to induce early genetic mutations and organ failures in humans on the premise of scientific advancement
 
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and the perils of synthetic biology.
 
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=== Content ===
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What are the public health risks imposed by the chemtrails agenda and why ? Is the spraying of biologically active aerosols over humans a delusional
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==== Dual inputs repressors and promoters: combinatorial transcription and amplification of arbitrary gene expression using dual promoters ====
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conspiracy theory or a landmark of organized crime to expose the human DNA to potentially harmful genetic material? Are chemtrails motivated by hate
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and anger of the human genome or by a eugenics movement dedicated to transforming the human DNA into profitable commercial products ?
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* single gene knock out
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The aim of this page is to collect data on so-called '''genetic switches''', or synthetic '''transcriptional devices''' and rationalize the evidences to justify the validity
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* Lac operon and LuxR ...
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of my concerns on the function of the chemtrails repeated spraying atop of my house and city, in Quebec and elsewhere.
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* just LuxR but no Lac ...
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* sugar (sucrose, fructose, galactose) etc..
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==== antibiotic resistance gene marking in genetically-modified corn (neomycin phosphotransferase II) ====
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=== Evidence collection ===
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*  http://brenda-enzymes.org/php/result_flat.php4?ecno=2.7.1.95
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==== Input/output promoters ====
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* http://metacyc.org/META/NEW-IMAGE?type=NIL&object=KANAMYCIN-KINASE-RXN&redirect=T
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* ATP = Adenosine triphosphate
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* reaction type is a "phosphotransferase" (A+B <=> C+D)
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* Enzyme possibly implicated in Leukemia disease (=> inducer of antibiotic resistance to [http://www.openwetware.org/wiki/Kanamycin Kanamycin]?)
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==== quorum sensing ====
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{| border="1" cellpadding="2" class="wikitable"
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|-
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! scope="col" | Promoter name
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! scope="col" | Promoter type
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! scope="col" | Description
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! scope="col" | Target organisms
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! scope="col" | Restriction site 
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! scope="col" | EC number
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! scope="col" | References
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! scope="col" | Biobrick Part
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|-
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! scope="row" | L-arabinose
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| aldopentose
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| Inducible pBAD/araC promoter
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| E.coli (K-12/MG1655), P.fluorescens(?), Homo sapiens
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| araBAD (''araC'')
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|
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| [http://openwetware.org/wiki/Arabinose 1], [http://en.wikipedia.org/wiki/Arabinose 2], [http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6976.2010.00226.x/abstract 3], [http://www.ncbi.nlm.nih.gov/pubmed/12080425?dopt=Abstract 4], [http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/regulation/AraC.html 5]
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| BBa_I0500
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|-
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! scope="row" | cAMP
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| second messenger
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| cyclic adenosine monophosphate is required to activate the Lac operon along with the araC promoter.
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| Homo sapiens
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| LacZ
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| 3.1.4.17, 3.1.4.53, 4.6.1.1
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| [http://en.wikipedia.org/wiki/Cyclic_adenosine_monophosphate 1]
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|   
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|}
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* GFP: can be grown on E. coli to transpose stuff on humans i guess (strain DH10B?)
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==== Plasmid addiction systems ====
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* UV-B test: positive correlation with the GFP marker (the organism is the product)
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=== Unsafe bare backing in the news: Get to know the people behind the scene ! ===
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{| border="1" cellpadding="2" class="wikitable"
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|-
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! scope="col" | Plasmid name
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! scope="col" | Origin of replication
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! scope="col" | Organism (Strain)
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! scope="col" | Anti-bacterial resistance
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! scope="col" | Quorum sensing protein expression 
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! scope="col" | ColE1 (Colicins) protein expression
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! scope="col" | 6-'''his'''-tagged promoter
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! scope="col" | References
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|-
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| scope="row" | pSB1A[C]3 (formerly pSB1A2?)
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| scope="row" | pUC19-derived (pMB1)
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| scope="row" | ''E.coli'' ([http://cgsc.biology.yale.edu/Strain.php?ID=111773 BW27783])
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| scope="row" | ampicilin (ampR)
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| scope="row" | Yes (GFP)
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| scope="row" | Yes (ccdB?)
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| scope="row" | Yes (L-arabinose inducible)
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| scope="row" | [http://partsregistry.org/Part:pSB1A2 1], [http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP 2], [http://partsregistry.org/Part:BBa_I746914 3], [http://partsregistry.org/Part:BBa_I0500 4], [http://partsregistry.org/wiki/index.php?title=Part:BBa_P1010 5]
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|-
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| scope="row" | pSB3K3-1010
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| scope="row" | p15a-derived (pMR101)
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| scope="row" | ''E.coli'' (MG1655)
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| scope="row" | kanamycin (kanR)
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| scope="row" | Yes (GFP/RFP via F2620)
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| scope="row" | Yes (ccdB?)
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| scope="row" | Yes (L-arabinose inducible)
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| [http://partsregistry.org/cgi/partsdb/related.cgi?part=F2620 1], [http://partsregistry.org/Part:BBa_F2620 2], [http://partsregistry.org/Part:BBa_V1000:Design 3]
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|}
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"Ginkgo BioWorks joins the Wyss Institute at Harvard University in $3.7M contract to develop a Genetic Security System for DARPA
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==== Reagents ====
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The Wyss Institute for Biologically Inspired Engineering at Harvard University announced today that it has been awarded a $3.7 million contract (including option) from the Defense Advanced Research Projects Agency (DARPA) to develop a genetic security system that would track an organism's history.
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[data here...]
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The proposed DNA-based memory device would sit inside a bacterium and create a permanent record of its historical experiences in much the same way as the "Track Changes" feature of word-processing software records successive edits in an electronic document. Such a bacterial background check would be analogous to biological forensic tools, such as fingerprint analysis, DNA testing, and blood typing.
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==== Discussion ====
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The DARPA project will be led by Wyss Institute core faculty member Pamela Silver, who is a professor of Systems Biology at Harvard Medical School and the first Director of Harvard's Program in Systems Biology. Co-Principal Investigators will be James Collins, also a Wyss Institute core faculty member as well as professor of Biomedical Engineering at Boston University, and Jason Kelly, a founder and Principal Scientist at the Boston-based startup Ginkgo Bioworks.
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*  A human is not a computer program and never will be. Love is not programmable and neither are emotions which makes life beautiful or harsh. It is through the eyes of natural-born scientists that humans may learn how to live in respect with the outer world and not the opposite. Yet I find scientific progress brilliant and at the same time horrified by the efforts made to confine the human species into a predictable sequence of digits. -- [[User:Etienne Robillard|Etienne Robillard]] 21:45, 15 October 2012 (EDT)
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* Whether the [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1681472/ T7.1 bacteriophage] was designed for E.coli or H.sapiens does not seem to limit its capacity to genetically modify (or [http://www.ncbi.nlm.nih.gov/pubmed/18410688 refactor]) DNA using a [http://www.ncbi.nlm.nih.gov/nuccore/169656098 F-plasmid like vector]. In bacterias, an equivalent process is known as [http://www.science-projects.com/F-sex.htm bacterial conjugation].
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The project team is charged with overseeing development of a bacterial memory system that actively reports on and tracks the history and status of an organism, providing information on its specific experiences, such as exposure to an antibiotic. This type of tracking system could protect commodity biomanufacturing by tracking the theft of proprietary bacterial strains that have been metabolically engineered to produce high-value products, such as biofuels or chemicals. It could also enhance the security of bacteria that are being studied in laboratory settings and discourage the misuse of dangerous biological pathogens.
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The device would need to be robust enough to function in the field, while also maintaining accurate historical records in the face of a wide range of environmental stresses, including the death of the bacterium that it is charged with tracking. "This would be one of the first DNA-based memory systems to accurately track bacteria and it represents just the kind of challenging -- and potentially game-changing -- work that we do best here at the Wyss Institute," said Wyss Institute Founding Director Donald Ingber, MD, Ph.D. "We are happy to collaborate with DARPA in creating a new way to help ensure that bacterial strains created for science and industry are not misused in ways that could harm people or endanger our access to important products.""
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Source: http://ginkgobioworks.com/news.html . More info about the founder of Ginkgo BioWorks [http://openwetware.org/wiki/Reshma_Shetty here].
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=== References ===
=== References ===
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<biblio>
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* http://dspace.mit.edu/handle/1721.1/41843
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#Shetty-JBiolEng-2008 pmid=18410688
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#Lutz-Bujard-Nucleic-Acid-Res-2007 pmid=9092630
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#Morgan-Kiss-PNAS-2002 pmid=12032290
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</biblio>
=== Keywords ===
=== Keywords ===
synthetic biology, biological engineering, transcriptional logic, transcription-based logic, engineered biological systems, PoPS, BioBrick standard biological part, eugenism, genocide, kill-switch, genetic switch, metabolic regulation, DNA programming, '''transcriptional inverter''', reverse engineering, deceit, collusion, scientific misconduct, scientific responsability, demagogy, ignorance, abuse of power, chemtrails, bare backing
synthetic biology, biological engineering, transcriptional logic, transcription-based logic, engineered biological systems, PoPS, BioBrick standard biological part, eugenism, genocide, kill-switch, genetic switch, metabolic regulation, DNA programming, '''transcriptional inverter''', reverse engineering, deceit, collusion, scientific misconduct, scientific responsability, demagogy, ignorance, abuse of power, chemtrails, bare backing

Revision as of 17:48, 22 October 2012

Project Bare Back Mountain: Decrypting the Eugenicist Connection hiden between the crypto-language of Synthetic Biology and Chemtrails

"For them, there is no God; the Government is the supreme principle deciding above all consideration of intangible moral. The family, seen as a "bourgeois institution" must be destroyed and replaced by a governmental control of reproduction and education, the Lebensborn. This is how the Government can control the "production" of human beings in the future, both in the quantitative and "qualitative" plan(8)." 1

Contents


Synopsis

What are the public health risks imposed by the chemtrails agenda and why ? Is the spraying of biologically active aerosols over humans a delusional conspiracy theory or a landmark of organized crime to expose the human DNA to potentially harmful genetic material? Are chemtrails motivated by hate and anger of the human genome or by a eugenics movement dedicated to transforming the human DNA into profitable commercial products ?

The aim of this page is to collect data on so-called genetic switches, or synthetic transcriptional devices and rationalize the evidences to justify the validity of my concerns on the function of the chemtrails repeated spraying atop of my house and city, in Quebec and elsewhere.

Evidence collection

Input/output promoters

Promoter name Promoter type Description Target organisms Restriction site EC number References Biobrick Part
L-arabinose aldopentose Inducible pBAD/araC promoter E.coli (K-12/MG1655), P.fluorescens(?), Homo sapiens araBAD (araC) 1, 2, 3, 4, 5 BBa_I0500
cAMP second messenger cyclic adenosine monophosphate is required to activate the Lac operon along with the araC promoter. Homo sapiens LacZ 3.1.4.17, 3.1.4.53, 4.6.1.1 1

Plasmid addiction systems

Plasmid name Origin of replication Organism (Strain) Anti-bacterial resistance Quorum sensing protein expression ColE1 (Colicins) protein expression 6-his-tagged promoter References
pSB1A[C]3 (formerly pSB1A2?) pUC19-derived (pMB1) E.coli (BW27783) ampicilin (ampR) Yes (GFP) Yes (ccdB?) Yes (L-arabinose inducible) 1, 2, 3, 4, 5
pSB3K3-1010 p15a-derived (pMR101) E.coli (MG1655) kanamycin (kanR) Yes (GFP/RFP via F2620) Yes (ccdB?) Yes (L-arabinose inducible) 1, 2, 3

Reagents

[data here...]

Discussion

  • A human is not a computer program and never will be. Love is not programmable and neither are emotions which makes life beautiful or harsh. It is through the eyes of natural-born scientists that humans may learn how to live in respect with the outer world and not the opposite. Yet I find scientific progress brilliant and at the same time horrified by the efforts made to confine the human species into a predictable sequence of digits. -- Etienne Robillard 21:45, 15 October 2012 (EDT)
  • Whether the T7.1 bacteriophage was designed for E.coli or H.sapiens does not seem to limit its capacity to genetically modify (or refactor) DNA using a F-plasmid like vector. In bacterias, an equivalent process is known as bacterial conjugation.

References

  1. Shetty RP, Endy D, and Knight TF Jr. . pmid:18410688. PubMed HubMed [Shetty-JBiolEng-2008]
  2. Lutz R and Bujard H. . pmid:9092630. PubMed HubMed [Lutz-Bujard-Nucleic-Acid-Res-2007]
  3. Morgan-Kiss RM, Wadler C, and Cronan JE Jr. . pmid:12032290. PubMed HubMed [Morgan-Kiss-PNAS-2002]
All Medline abstracts: PubMed HubMed

Keywords

synthetic biology, biological engineering, transcriptional logic, transcription-based logic, engineered biological systems, PoPS, BioBrick standard biological part, eugenism, genocide, kill-switch, genetic switch, metabolic regulation, DNA programming, transcriptional inverter, reverse engineering, deceit, collusion, scientific misconduct, scientific responsability, demagogy, ignorance, abuse of power, chemtrails, bare backing

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