User:Etienne Robillard/Notebook/chim trills notebook/ForensicEvidencesChart

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(Plasmid addiction systems)
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==== Plasmid addiction systems ====
==== Plasmid addiction systems ====
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"BioBrick vector names take the form pSB#X#. The letters pSB are an acronym for plasmid Synthetic Biology. The first number denotes the origin of replication (Table ​(Table2).2). The letter X identifies the antibiotic resistance marker(s) present in the vector (Table ​(Table3).3). Vectors with multiple resistance markers have multiple, successive letters. Finally, the last number in the vector name is a version number to differentiate between the various implementations of the pSB series of vectors (Table ​(Table4).4). When referring to both a BioBrick standard biological part and the vector in which it is cloned, the convention is to use the form [vector name]- [part number] such as pSB4K5-T9003. To refer to BioBrick vectors to be used for construction of BioBrick parts, use the full vector name and default cloned part. For example, pSB4A3-P1010, pSB1A10-P1010, pSB4K5-I52002, and pSB3T5-I52001 are all available vectors from the Registry of Standard Biological Parts. However, for convenience, vector names are often abbreviated to pSB4A3, pSB1A10, pSB4K5, and pSB3T5, respectively. New plasmid-based vectors constructed from the BioBrick base vector BBa_I51020 should be named pSB#X5-I52002 where the # is determined by the identity of the replication origin and the letter X is determined by the antibiotic resistance marker(s) present. To expand the BioBrick vector nomenclature, submit new vectors or vector parts to the Registry of Standard Biological Parts and document any new annotation needed [66]. The BioBricks Foundation is leading an open standards setting process should any revisions to the BioBrick vector nomenclature beyond addition of new replication origins, antibiotic resistance markers and version numbers be needed." [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373286 1]
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{| border="1" cellpadding="2" class="wikitable"
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|-
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! scope="col" | Plasmid name
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! scope="col" | Origin of replication
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! scope="col" | Organism (Strain)
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! scope="col" | Anti-bacterial resistance
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! scope="col" | Quorum sensing protein expression 
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! scope="col" | ColE1 (Colicins) protein expression
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! scope="col" | 6-'''his'''-tagged promoter
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! scope="col" | References
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|-
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| scope="row" | pSB1A[C]3 (formerly pSB1A2?)
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| scope="row" | pUC19-derived (pMB1)
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| scope="row" | ''E.coli'' ([http://cgsc.biology.yale.edu/Strain.php?ID=111773 BW27783])
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| scope="row" | ampicilin (ampR)
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| scope="row" | Yes (GFP)
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| scope="row" | Yes (ccdB?)
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| scope="row" | Yes (L-arabinose inducible)
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| scope="row" | [http://partsregistry.org/Part:pSB1A2 1], [http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP 2], [http://partsregistry.org/Part:BBa_I746914 3], [http://partsregistry.org/Part:BBa_I0500 4], [http://partsregistry.org/wiki/index.php?title=Part:BBa_P1010 5]
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|-
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| scope="row" | pSB3K3-1010
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| scope="row" | p15a-derived (pMR101)
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| scope="row" | ''E.coli'' (MG1655)
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| scope="row" | kanamycin (kanR)
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| scope="row" | Yes (GFP/RFP via F2620)
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| scope="row" | Yes (ccdB?)
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| scope="row" | Yes (L-arabinose inducible)
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| [http://partsregistry.org/cgi/partsdb/related.cgi?part=F2620 1], [http://partsregistry.org/Part:BBa_F2620 2], [http://partsregistry.org/Part:BBa_V1000:Design 3]
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|}
==== Reagents ====
==== Reagents ====
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*  A human is not a computer program and never will be. Love is not programmable and neither are emotions which makes life beautiful or harsh. It is through the eyes of natural-born scientists that humans may learn how to live in respect with the outer world and not the opposite. Yet I find scientific progress brilliant and at the same time horrified by the efforts made to confine the human species into a predictable sequence of digits. -- [[User:Etienne Robillard|Etienne Robillard]] 21:45, 15 October 2012 (EDT)
*  A human is not a computer program and never will be. Love is not programmable and neither are emotions which makes life beautiful or harsh. It is through the eyes of natural-born scientists that humans may learn how to live in respect with the outer world and not the opposite. Yet I find scientific progress brilliant and at the same time horrified by the efforts made to confine the human species into a predictable sequence of digits. -- [[User:Etienne Robillard|Etienne Robillard]] 21:45, 15 October 2012 (EDT)
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* Whether the T7 bacteriophage was designed for E.coli or H.sapiens does not seem to limit its capacity to genetically modify (or refactor) DNA using a [http://www.ncbi.nlm.nih.gov/nuccore/169656098 F-plasmid like vector]. In bacterias, an equivalent process is known as [http://www.science-projects.com/F-sex.htm bacterial conjugation].
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* Whether the [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1681472/ T7.1 bacteriophage] was designed for E.coli or H.sapiens does not seem to limit its capacity to genetically modify (or [http://www.ncbi.nlm.nih.gov/pubmed/18410688 refactor]) DNA using a [http://www.ncbi.nlm.nih.gov/nuccore/169656098 F-plasmid like vector]. In bacterias, an equivalent process is known as [http://www.science-projects.com/F-sex.htm bacterial conjugation].
=== References ===
=== References ===
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<biblio>
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#Shetty-JBiolEng-2008 pmid=18410688
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#Lutz-Bujard-Nucleic-Acid-Res-2007 pmid=9092630
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#Morgan-Kiss-PNAS-2002 pmid=12032290
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</biblio>
=== Keywords ===
=== Keywords ===
synthetic biology, biological engineering, transcriptional logic, transcription-based logic, engineered biological systems, PoPS, BioBrick standard biological part, eugenism, genocide, kill-switch, genetic switch, metabolic regulation, DNA programming, '''transcriptional inverter''', reverse engineering, deceit, collusion, scientific misconduct, scientific responsability, demagogy, ignorance, abuse of power, chemtrails, bare backing
synthetic biology, biological engineering, transcriptional logic, transcription-based logic, engineered biological systems, PoPS, BioBrick standard biological part, eugenism, genocide, kill-switch, genetic switch, metabolic regulation, DNA programming, '''transcriptional inverter''', reverse engineering, deceit, collusion, scientific misconduct, scientific responsability, demagogy, ignorance, abuse of power, chemtrails, bare backing

Revision as of 16:48, 22 October 2012

Project Bare Back Mountain: Decrypting the Eugenicist Connection hiden between the crypto-language of Synthetic Biology and Chemtrails

"For them, there is no God; the Government is the supreme principle deciding above all consideration of intangible moral. The family, seen as a "bourgeois institution" must be destroyed and replaced by a governmental control of reproduction and education, the Lebensborn. This is how the Government can control the "production" of human beings in the future, both in the quantitative and "qualitative" plan(8)." 1

Contents


Synopsis

What are the public health risks imposed by the chemtrails agenda and why ? Is the spraying of biologically active aerosols over humans a delusional conspiracy theory or a landmark of organized crime to expose the human DNA to potentially harmful genetic material? Are chemtrails motivated by hate and anger of the human genome or by a eugenics movement dedicated to transforming the human DNA into profitable commercial products ?

The aim of this page is to collect data on so-called genetic switches, or synthetic transcriptional devices and rationalize the evidences to justify the validity of my concerns on the function of the chemtrails repeated spraying atop of my house and city, in Quebec and elsewhere.

Evidence collection

Input/output promoters

Promoter name Promoter type Description Target organisms Restriction site EC number References Biobrick Part
L-arabinose aldopentose Inducible pBAD/araC promoter E.coli (K-12/MG1655), P.fluorescens(?), Homo sapiens araBAD (araC) 1, 2, 3, 4, 5 BBa_I0500
cAMP second messenger cyclic adenosine monophosphate is required to activate the Lac operon along with the araC promoter. Homo sapiens LacZ 3.1.4.17, 3.1.4.53, 4.6.1.1 1

Plasmid addiction systems

Plasmid name Origin of replication Organism (Strain) Anti-bacterial resistance Quorum sensing protein expression ColE1 (Colicins) protein expression 6-his-tagged promoter References
pSB1A[C]3 (formerly pSB1A2?) pUC19-derived (pMB1) E.coli (BW27783) ampicilin (ampR) Yes (GFP) Yes (ccdB?) Yes (L-arabinose inducible) 1, 2, 3, 4, 5
pSB3K3-1010 p15a-derived (pMR101) E.coli (MG1655) kanamycin (kanR) Yes (GFP/RFP via F2620) Yes (ccdB?) Yes (L-arabinose inducible) 1, 2, 3

Reagents

[data here...]

Discussion

  • A human is not a computer program and never will be. Love is not programmable and neither are emotions which makes life beautiful or harsh. It is through the eyes of natural-born scientists that humans may learn how to live in respect with the outer world and not the opposite. Yet I find scientific progress brilliant and at the same time horrified by the efforts made to confine the human species into a predictable sequence of digits. -- Etienne Robillard 21:45, 15 October 2012 (EDT)
  • Whether the T7.1 bacteriophage was designed for E.coli or H.sapiens does not seem to limit its capacity to genetically modify (or refactor) DNA using a F-plasmid like vector. In bacterias, an equivalent process is known as bacterial conjugation.

References

  1. Shetty RP, Endy D, and Knight TF Jr. . pmid:18410688. PubMed HubMed [Shetty-JBiolEng-2008]
  2. Lutz R and Bujard H. . pmid:9092630. PubMed HubMed [Lutz-Bujard-Nucleic-Acid-Res-2007]
  3. Morgan-Kiss RM, Wadler C, and Cronan JE Jr. . pmid:12032290. PubMed HubMed [Morgan-Kiss-PNAS-2002]
All Medline abstracts: PubMed HubMed

Keywords

synthetic biology, biological engineering, transcriptional logic, transcription-based logic, engineered biological systems, PoPS, BioBrick standard biological part, eugenism, genocide, kill-switch, genetic switch, metabolic regulation, DNA programming, transcriptional inverter, reverse engineering, deceit, collusion, scientific misconduct, scientific responsability, demagogy, ignorance, abuse of power, chemtrails, bare backing

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