User:Federico Castro M/Projects/VSFH

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(Data: RNA genome size limit.)
(Data: 50kb RNA genome.)
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Concivably an organism could survive in a controlled environment with a minimal genome of just 113kbp and 151 genes ([http://www.nature.com/msb/journal/v2/n1/full/msb4100090.html Forster 2006]). However, recent estimates sugest that LUCA was complex and probably had more than 600 genes ([http://www.informatics.sussex.ac.uk/users/ctf20/dphil_2005/CSNs/Minimal%20Genome/nrmicro751.pdf Kooning 2003]).
Concivably an organism could survive in a controlled environment with a minimal genome of just 113kbp and 151 genes ([http://www.nature.com/msb/journal/v2/n1/full/msb4100090.html Forster 2006]). However, recent estimates sugest that LUCA was complex and probably had more than 600 genes ([http://www.informatics.sussex.ac.uk/users/ctf20/dphil_2005/CSNs/Minimal%20Genome/nrmicro751.pdf Kooning 2003]).
* Check if a RNA genome can sustain such genome size.
* Check if a RNA genome can sustain such genome size.
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RNA is much more error-prone than DNA due to its higher mutation rate, so that RNA molecules cannot exceed certain size (Eigen limit) without falling into replicative catastrophe. ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC129678/?tool=pubmed Eigen 2002])
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RNA is much more error-prone than DNA due to its higher mutation rate, so that RNA molecules cannot exceed certain size (Eigen limit) without falling into replicative catastrophe ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC129678/?tool=pubmed Eigen 2002]). This limit is around 50kb which is only enough for a dozen genes or so.
* Does it had the necesary genetic machinery to support DNA?
* Does it had the necesary genetic machinery to support DNA?

Revision as of 15:33, 10 November 2009

This project is about designing an organism that resembles the Last Universal Common Ancestor and whose construction is feasible.

Replication machinery

We have to start by:

  • Estimate the genomic size of LUCA.

Concivably an organism could survive in a controlled environment with a minimal genome of just 113kbp and 151 genes (Forster 2006). However, recent estimates sugest that LUCA was complex and probably had more than 600 genes (Kooning 2003).

  • Check if a RNA genome can sustain such genome size.

RNA is much more error-prone than DNA due to its higher mutation rate, so that RNA molecules cannot exceed certain size (Eigen limit) without falling into replicative catastrophe (Eigen 2002). This limit is around 50kb which is only enough for a dozen genes or so.

  • Does it had the necesary genetic machinery to support DNA?

Atte (membrillo): Wweww Desde Xicotencatl Tlalpan, translate this Google :)

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