User:Federico Castro M/Projects/VSFH

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(Data: 50kb RNA genome.)
(Data: DNA genetic machinery was present in LUCA.)
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* Check if a RNA genome can sustain such genome size.
* Check if a RNA genome can sustain such genome size.
RNA is much more error-prone than DNA due to its higher mutation rate, so that RNA molecules cannot exceed certain size (Eigen limit) without falling into replicative catastrophe ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC129678/?tool=pubmed Eigen 2002]). This limit is around 50kb which is only enough for a dozen genes or so.
RNA is much more error-prone than DNA due to its higher mutation rate, so that RNA molecules cannot exceed certain size (Eigen limit) without falling into replicative catastrophe ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC129678/?tool=pubmed Eigen 2002]). This limit is around 50kb which is only enough for a dozen genes or so.
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* Does it had the necesary genetic machinery to support DNA?
+
* Did LUCA have the necesary genetic machinery to support DNA?
 +
Yes, three conserved genes have been found that were asociated with transcription and replication of DNA ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC430263/ Harris 2002]).
<span style="color:white">Atte (membrillo): Wweww
<span style="color:white">Atte (membrillo): Wweww
Desde Xicotencatl Tlalpan, translate this Google :)
Desde Xicotencatl Tlalpan, translate this Google :)
</span>
</span>

Revision as of 16:50, 10 November 2009

This project is about designing an organism that resembles the Last Universal Common Ancestor and whose construction is feasible.

Replication machinery

We have to start by:

  • Estimate the genomic size of LUCA.

Concivably an organism could survive in a controlled environment with a minimal genome of just 113kbp and 151 genes (Forster 2006). However, recent estimates sugest that LUCA was complex and probably had more than 600 genes (Kooning 2003).

  • Check if a RNA genome can sustain such genome size.

RNA is much more error-prone than DNA due to its higher mutation rate, so that RNA molecules cannot exceed certain size (Eigen limit) without falling into replicative catastrophe (Eigen 2002). This limit is around 50kb which is only enough for a dozen genes or so.

  • Did LUCA have the necesary genetic machinery to support DNA?

Yes, three conserved genes have been found that were asociated with transcription and replication of DNA (Harris 2002).

Atte (membrillo): Wweww Desde Xicotencatl Tlalpan, translate this Google :)

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