WORK B1
1st LSI-CBR Design of Experiment (DOE) process Optimization Workshop
Teaching:
When
April 5th - April 14th 2011
Where
LSI 4th floor CBR Fermentation Suite (Rm 4332) and Rm 4332
Attending:
- Louise Creagh: Senior Instructor, Manager at Centre for BioThermodynamics, Laboratory of Molecular Biophysics
- Raymond Pan: PhD Student, Department of Biochemistry and Molecular Biology(Bromme lab)
- Swati Yewlakar: Postdoctoral Fellow, Department of Chemical and Biological Engineering
- Tacky Loonchanta: PhD Student, Department of Chemistry (Burtnick lab)<-- Decision pending
Off-line Measurement
Day
|
Group
|
Run
|
Temp
|
DO
|
pH
|
Day
|
OD
|
Media
|
Day
|
D-1
|
D-2
|
D-3
|
'
|
Cell growth
|
Phage
|
Yield of Phage
|
-1 |
I |
1 |
28.5 |
70.0 |
6.5 |
0 |
(OD= ) |
( ml) |
1 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
-1 |
I |
2 |
28.5 |
70.0 |
10.7 |
0 |
(OD= ) |
( ml) |
1 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
-1 |
I |
3 |
37.0 |
100.0 |
4.0 |
0 |
(OD= ) |
( ml) |
1 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
1 |
A |
4 |
14.2 |
70.0 |
6.5 |
2 |
(OD= ) |
( ml) |
3 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
1 |
A |
5 |
28.5 |
70.0 |
6.5 |
2 |
(OD= ) |
( ml) |
3 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
1 |
A |
6 |
20.0 |
100.0 |
4.0 |
2 |
(OD= ) |
( ml) |
3 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
2 |
B |
7 |
28.5 |
120.5 |
6.5 |
3 |
(OD= ) |
( ml) |
4 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
2 |
B |
8 |
20.0 |
100.0 |
9.0 |
3 |
(OD= ) |
( ml) |
4 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
2 |
B |
9 |
20.0 |
40.0 |
4.0 |
3 |
(OD= ) |
( ml) |
4 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
3 |
A |
10 |
28.5 |
70.0 |
6.5 |
4 |
(OD= ) |
( ml) |
5 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
3 |
A |
11 |
42.8 |
70.0 |
6.5 |
4 |
(OD= ) |
( ml) |
5 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
3 |
A |
12 |
37.0 |
100.0 |
9.0 |
4 |
(OD= ) |
( ml) |
5 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
6 |
B |
13 |
28.5 |
70.0 |
6.5 |
7 |
(OD= ) |
( ml) |
8 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
6 |
B |
14 |
28.5 |
19.5 |
6.5 |
7 |
(OD= ) |
( ml) |
8 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
6 |
B |
15 |
20.0 |
40.0 |
9.0 |
7 |
(OD= ) |
( ml) |
8 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
7 |
A |
16 |
28.5 |
70.0 |
2.3 |
8 |
(OD= ) |
( ml) |
9 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
7 |
A |
17 |
37.0 |
40.0 |
9.0 |
8 |
(OD= ) |
( ml) |
9 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
7 |
A |
18 |
28.5 |
70.0 |
6.5 |
8 |
(OD= ) |
( ml) |
9 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
8 |
B |
19 |
37.0 |
40.0 |
4.0 |
9 |
(OD= ) |
( ml) |
10 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
8 |
B |
20 |
28.5 |
70.0 |
6.5 |
9 |
(OD= ) |
( ml) |
10 |
(R1D1:data) |
(R1D2:data) |
(R1D3:data) |
|
(cell grams) |
(TU/total) |
(TU/grams)
|
|
Day 1 (April 5th, Tue)
Runs
|
1, 2, 3
|
4, 5, 6
|
7, 8, 9
|
07:30 |
|
SH: Prepare hight density K19 cells |
|
08:00 |
|
SH: Prepare dilution tubes |
|
09:00 |
Ann: Colony check |
Group A: Sampling |
|
09:30 |
|
Ann: Phage dilution |
Group B: Assemble bioreactors
|
09:50 |
|
Ann: OD of HD cells |
Group B: Autoclve
|
10:00 |
|
Ann: Dry plates |
|
10:30 |
|
Ann: Infect K19 cells |
|
11:10 |
|
Ann: Spot titer |
Group B: Install bioreactors
|
15:30 |
|
|
SH: Thaw stock
|
16:50 |
|
|
SH: Pre-culture for HD K19 cells
|
17:00 |
|
|
SH: Inoculation
|
|
10:00 - 11:00 Presentation (Rm 4.303)
# Fermentation Database and Websites
# How to Install Bioreactors
# How to Setup Bio-controllers
Day 1 (April 5th, Tue)
Runs
|
'
|
1, 2, 3
|
4, 5, 6
|
07:30 |
|
SH: Prepare hight density K19 cells |
|
08:00 |
|
SH: Prepare dilution tubes |
|
09:00 |
|
Group A: Sampling |
|
09:30 |
|
Ann: Phage dilution |
Group B: Assemble bioreactors
|
09:50 |
|
Ann: OD of HD cells |
Group B: Autoclve
|
10:00 |
|
Ann: Dry plates |
|
10:30 |
|
Ann: Infect K19 cells |
|
11:10 |
|
Ann: Spot titer |
Group B: Install bioreactors
|
15:30 |
|
|
SH: Thaw stock
|
16:50 |
|
|
SH: Pre-culture for HD K19 cells
|
17:00 |
|
|
SH: Inoculation
|
|
10:00 - 11:00 Presentation (Rm 4.303)
# Fermentation Database and Websites
# How to Install Bioreactors
# How to Setup Bio-controllers
Day 3 (April 7th, Thu)
Runs
|
4, 5, 6
|
7, 8, 9
|
10, 11, 12
|
07:30 |
|
SH: Prepare hight density K19 cells |
|
08:00 |
|
SH: Prepare dilution tubes |
|
09:00 |
Group A: Colony check |
Group B: Sampling |
|
09:30 |
|
Ann: Phage dilution |
Group A: Assemble bioreactors
|
09:50 |
|
Ann: OD of HD cells |
Group A: Autoclve
|
10:00 |
|
Ann: Dry plates |
|
10:30 |
|
Ann: Infect K19 cells |
|
11:10 |
|
Ann: Spot titer |
Group A: Install bioreactors
|
15:30 |
|
|
SH: Thaw stock
|
16:50 |
|
|
SH: Pre-culture for HD K19 cells
|
17:00 |
|
|
SH: Inoculation
|
|
10:00 - 11:00 Presentation (Rm 4.303)
# DOE Step 1. Design Experiments
Helful links
- http://meta.wikimedia.org/wiki/Table
- http://excel2wiki.net/ <--- Use this (Copy and Paste Excel to wiki converter)!
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